Summary
A system has been developed for rapid selection of streptomycin resistant mutants, as adventitious shoots arising from explants of several Solanaceous species. Efficient mutagenesis was achieved by incubating shoot culture-derived leaf strips with 1 or 5 mM nitroso-methylurea, for 90 or 120 min. In Nicotiana tabacum and Lycopersicon peruvianum these treatments resulted in white or variegated adventitious shoots from up to 3.5% of explants placed on medium promoting shoot regeneration. Chlorophyll deficiencies were only observed very rarely in Solanum nigrum. Streptomycin resistant shoots were obtained from leaf explants placed on medium containing 500 mg l-1 streptomycin sulphate, under which conditions explants are bleached and adventitious shoot development suppressed. Green adventitious s shoots appeared at a frequency dependent both on the mutagenic treatment and on the species. The best response was with S. nigrum where >70% of the explants produced streptomycin resistant shoots, most of which retained their resistance on subsequent testing. Maternal inheritance of streptomycin resistance has been confirmed for several N. tabacum and S. nigrum mutants, and there is also evidence for paternal transmission in the latter species. The procedure has been successfully extended to other species, including N. sylvestris and N. plumbaginifolia, and also to obtain spectinomycin resistant mutants.
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References
Arntzen CJ, Duesing JH (1984) Chloroplast-encoded herbicide resistance. In: Downey K, Voellmy RW, Ahmad F, Shultz J (eds) Advances in gene technology: molecular genetics of plants and animals (Miami Winter Symposia, vol 20) Academic Press, New York, pp 273–294
Carter BLA, Dawes IW (1978) Nitrosoguanidine mutagenesis during the yeast cell cycle. Mutat Res 51:289–292
Cséplö A, Maliga P (1982) Lincomycin resistance, a new type of maternally inherited mutation in Nicotiana plumbaginifolia. Curr Genet 6:105–109
Cséplö A, Maliga P (1984) Large scale isolation of maternally inherited lincomycin resistance mutations in diploid Nicotiana plumbaginifolia protoplast cultures. Mol Gen Genet 196:407–412
Cséplö A, Medgyesy P, Hideg, E, Demeter S, Márton L, Maliga P (1985) Triazine-resistant Nicotiana mutants from photomixotrophic cell cultures. Mol Gen Genet 200:508–510
Day A, Ellis THN (1984) Chloroplast DNA deletions associated with wheat plants regenerated from pollen: possible basis for maternal inheritance of chloroplasts. Cell 39:359–368
Day A, Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Curr Genet 9:671–678
Dix PJ (1986) Cell line selection. In: Yeoman MM (ed) Plant cell culture technology. Blackwell Scientific Publications, London, pp 143–201
Epp MD (1973) Nuclear gene-induced plastome mutations in Oenothera hookeri. I. Genetic analysis. Genetics 75:465–483
Fluhr R, Aviv D, Galun E, Edleman M (1985) Eflicient induction and selection of chloroplast-encoded antibiotic resistant mutants in Nicotiana. Proc Natl Acad Sci USA 82:1485–1489
Gasquez J, Darmency H, Compoint J-P (1981) Étude de la transmission de la résistance chloroplastique aux triazines chez Solanum nigrum L. C R Acad Sci Paris t. 292:847–849
Hagemann R (1982) Induction of plastome mutations by nitrosourea compounds. In: Edelman M, Hallick RB, Chua N-H (eds) Methods in chloroplast molecular biology. Elsevier Biomedical Press, Amsterdam, pp 119–127
Hamill J, Ahuja PS, Davey MR, Cocking EC (1986) Protoplast-derived streptomycin-resistant plants of the forage legume Onobrychis viciifolia Scop. (sainfoin). Plant Cell Reports 5:439–441
Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rodgers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231
Hosticka LP, Hanson MR (1984) Induction of plastid mutations in tomatoes by nitrosomethylurea. J Hered 75:242–246
King PJ (1984) Mutagenesis of cultured cells. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, New York, pp 547–551
Maliga P (1984) Cell culture procedures for mutant selection and characterization in Nicotiana plumbaginifolia. In Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, New York, pp 552–562
Maliga P, Sz-Breznovits A, Márton L (1973) Streptomycin resistant plants from callus culture of haploid tobacco. Nature New Biol 244:28–30
Maliga P, Lörz H, Lázár G, Nagy F (1982) Cytoplast-protoplast fusion for interspecific chloroplast transfer in Nicotiana. Mol Gen Genet 185:211–215
Medgyesy P, Fejes E, Maliga P (1985) Interspecific chloroplast recombination in a Nicotiana somatic hybrid. Proc Natl Acad Sci USA 82:6969–6964
Medgyesy P, Páy A, Márton L (1986) Transmission of paternal chloroplasts in Nicotiana. Mol Gen Genet 204:195–198
Menczel L, Polsby LS, Steinback KE, Maliga P (1986) Fusionmediated transfer of triazine-resistant chloroplasts: Characterization of Nicotiana tabacum cybrid plants. Mol Gen Genet 205:201–205
Meredith CP (1979) Shoot development in established callus cultures of tomato (Lycopersicon esculentum Mill.). Z Pflanzenphysiol 95:405–411
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Sváb Z, Maliga P (1986) Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency. Theor Appl Genet 72:637–643
Umiel N (1979) Streptomycin resistance in tobacco: III. A test on germinating seedlings indicates cytoplasmic inheritance in the St-R701 mutant. Z Pflanzenphysiol 93:295–301
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Cabe, P.F.M., Timmons, A.M. & Dix, P.J. A simple procedure for the isolation of streptomycin resistant plants in Solanaceae . Mol Gen Genet 216, 132–137 (1989). https://doi.org/10.1007/BF00332241
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DOI: https://doi.org/10.1007/BF00332241