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Doubled haploids of novel trigenomic Brassica derived from various interspecific crosses

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Abstract

To develop doubled haploid (DH) mapping populations of hexaploid Brassica, 10 F1 hybrids derived from crosses between allohexaploid Brassica parents were evaluated in this study. The allohexaploid Brassica parents were selfed progenies of unique interspecific crosses between Brassica rapa (genome AA) × B. carinata (BBCC), B. nigra (BB) × B. napus (AACC), and a complex cross between B. juncea (AABB), B. napus and B. carinata, with relatively stable chromosome number (2n = 54). Hexaploid status and chromosome behavior during meiosis I in four promising F1 hybrids were assessed using microscopy and flow cytometry, and progeny were obtained following microspore culture. Hybrids H11-2 and H16-1 demonstrated higher amenability for embryo generation, plantlet regeneration, and frequency of production of DH microspore-derived progeny of hexaploid DNA content (6x) compared to hybrids H08-1 and H24-1. A total of 370 6x DH progeny were selected out of 693 plantlets from H11-2, 241/436 from H16-1, 23/54 from H08-1, and 21/56 from H24-1. DH progenies of hybrids H11-2 and H16-1 were then designated as promising mapping populations of a new hexaploid Brassica species.

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Abbreviations

ANOVA:

Analysis of variance

DH:

Doubled haploid

MD:

Microspore-derived

PMC:

Pollen mother cells

PI:

Propidium iodide

RFLP:

Restriction fragment length polymorphism

SSR:

Simple sequence repeats

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Acknowledgments

This work was supported partly by the Science and Technology Department of Zhejiang Province (2012C12902-1, 2011R50026-5), the National Natural Science Foundation of China (31000678), and the Australia-China Special Fund, jointly managed by the Department of Innovation Industry Science and Research (Australia) and the Ministry of Science and Technology, National Natural Science Foundation (China). I.A. Astarini was the recipient of an Indonesian government Program of Academic Recharging (PAR). We thank Anouska Cousin for helping in microspore culture procedures.

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Author notes

  1. A. S. Mason

    Present address: Centre for Integrative Legume Research and School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia

Authors and Affiliations

  1. Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China

    X. X. Geng & W. J. Zhou

  2. School of Plant Biology, The University of Western Australia, Crawley, WA, 6009, Australia

    X. X. Geng, S. Chen, I. A. Astarini, G. J. Yan, M. N. Nelson, A. S. Mason & A. Pradhan

  3. The UWA Institute of Agriculture, The University of Western Australia, Crawley, WA, 6009, Australia

    S. Chen, G. J. Yan, M. N. Nelson & W. A. Cowling

  4. Universitas Udayana, Kampus Bukit Jimbaran, Bali, Indonesia

    I. A. Astarini

  5. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    E. Tian, J. Meng, Z. Y. Li & X. H. Ge

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  1. X. X. Geng
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  6. J. Meng
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Corresponding author

Correspondence to W. J. Zhou.

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X. X. Geng and S. Chen: equal first authors.

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Geng, X.X., Chen, S., Astarini, I.A. et al. Doubled haploids of novel trigenomic Brassica derived from various interspecific crosses. Plant Cell Tiss Organ Cult 113, 501–511 (2013). https://doi.org/10.1007/s11240-013-0292-4

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  • DOI: https://doi.org/10.1007/s11240-013-0292-4

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