Plant Molecular Biology Reporter

, Volume 31, Issue 3, pp 517–523

The Negative Regulator OsSDS1 Significantly Reduces Salt and Drought Tolerance in Transgenic Arabidopsis

  • Guoyun Xu
  • Mingjuan Li
  • Mei Huang
  • Yanchun Cui
  • Manling Wang
  • Xinjie Xia
Original Paper

DOI: 10.1007/s11105-012-0521-8

Cite this article as:
Xu, G., Li, M., Huang, M. et al. Plant Mol Biol Rep (2013) 31: 517. doi:10.1007/s11105-012-0521-8
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Abstract

In this report, we present data on OsSDS1 (Oryza sativa L. salt and drought sensitive gene 1)—an uncharacterized gene isolated from rice Pei’ai 64S (O. sativa L.). Expression of OsSDS1 was strongly up-regulated by a wide spectrum of stresses, including cold, drought, and heat, in different tissues at different developmental stages of rice, as revealed by both microarray and quantitative RT-PCR analyses. Subcellular localization revealed that an OsSDS1: GFP fusion protein was distributed to the nucleus. Expression of OsSDS1 conferred decreased tolerance to salt and drought in Arabidopsis thaliana, accompanied by altered expression of stress-responsive genes and altered K+/Na+ ratio. The results suggest that OsSDS1 may act as a negative regulator of salt and drought tolerance in plants.

Keywords

ArabidopsisOsSDS1SaltDroughtOverexpression

Abbreviations

CaMV

Cauliflower mosaic virus

GFP

Green fluorescent protein

Pn

Net photosynthesis rate

qRT-PCR

Quantitative real-time RT-PCR

WT

Wild type

Supplementary material

11105_2012_521_MOESM1_ESM.doc (66 kb)
ESM 1(DOC 65 kb)

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Guoyun Xu
    • 1
  • Mingjuan Li
    • 1
  • Mei Huang
    • 1
  • Yanchun Cui
    • 1
  • Manling Wang
    • 1
  • Xinjie Xia
    • 1
  1. 1.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of SciencesHunanChina