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Different effects of DEP1 on vascular bundle- and panicle-related traits under indica and japonica genetic backgrounds

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Abstract

Rice (Oryza sativa L.) is a major food for more than half of the world’s population and serves as a model monocot plant. DEP1 (dense and erect panicle 1), corresponding to an erect-panicle architecture, may bring the third breakthrough in rice breeding after the introduction of the semi-dwarf trait and the application of heterosis in hybrid rice. The aim of this study was to determine the effects of DEP1 on yield components and vascular bundle-related traits under indica and japonica genetic backgrounds. We analyzed a series of recombinant inbred lines, which were derived from a cross between the japonica variety SN265 with an erect panicle and the indica variety R99 with a curved panicle. The results showed that effects of the DEP1/dep1 allele were much stronger than the effects of the indica/japonica genetic background on vascular bundle-related traits and yield components. As the frequency of indica alleles increased, the grain yield per plant and the harvest index significantly increased in lines with the dep1 allele. Among the indica-type lines, those with the dep1 allele showed superior values for almost all of the vascular bundle-related traits and yield components. Our results provide new insights into the use of a high-yield-related gene derived from japonica in the indica genetic background.

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Acknowledgments

This work was supported by Grants from the National Natural Science Foundation of China (No. 31430062 and 31371587) and a class general financial Grant from the China Postdoctoral Science Foundation (Grant No. 2014M560211, Postdoctoral No. 142541).

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    Authors and Affiliations

    1. Rice Research Institute of Shenyang Agricultural University/Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education and Liaoning Province/Key Laboratory of Northeast Rice Biology and Genetics and Breeding, Ministry of Agriculture, Shenyang, 110866, China

      Quan Xu, Tiansheng Liu, Wenjing Bi, Yuanzheng Wang, Hai Xu, Liang Tang & Zhengjin Xu

    2. Rice Applied Genomics Research Unit, Agrogenomics Research Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan

      Jian Sun

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    1. Quan Xu
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    2. Tiansheng Liu
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    3. Wenjing Bi
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    4. Yuanzheng Wang
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    5. Hai Xu
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    Correspondence to Quan Xu.

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    Quan Xu and Tiansheng Liu have contributed equally to this work.

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    Figure S1

    DEP1 allele in R99 and SN265. (a) Schematic representation of DEP1 allele in R99 and SN265. Light gray boxes and dark gray boxes represent UTR region and exons, respectively. Lines separating boxes represent introns. (b) Amino acid sequences of DEP1 alleles in R99 and SN265, gray boxes indicate same alignment. (PPTX 82 kb)

    Figure S2

    Linkage map of INDEL and SSILP subspecies-specific markers used in this study (PPTX 136 kb)

    Figure S3

    Relationship between V/R ratio and frequency of indica allele markers in curved- and erect-panicle lines (PPTX 59 kb)

    Supplementary material 4 (XLSX 17 kb)

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    Xu, Q., Liu, T., Bi, W. et al. Different effects of DEP1 on vascular bundle- and panicle-related traits under indica and japonica genetic backgrounds. Mol Breeding 35, 173 (2015). https://doi.org/10.1007/s11032-015-0364-8

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