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Molecular endocrine changes of Gh/Igf1 axis in gilthead sea bream (Sparus aurata L.) exposed to different environmental salinities during larvae to post-larvae stages

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Abstract

The influence of acclimation of the euryhaline gilthead sea bream (Sparus aurata) larvae/post-larvae to brackish water on growth, energetic contents, and mRNA levels of selected hormones and growth-regulating hypothalamic neurohormones was assessed. Specimens from 49 days post-hatching were acclimated during 28 days to two different environmental salinities: 38 and 20 psu (as brackish water). Both groups were then transferred to 38 psu and acclimated for an additional week. Early juveniles were sampled after 28 days of acclimation to both salinities and one week after transfer to 38 psu. Pituitary adenylate cyclase-activating peptide (adcyap1; pacap), somatostatin-I (sst1), growth hormone (gh1), insulin-like growth factor-I (igf1), and prolactin (prl) mRNA expression were all studied by QPCR. Post-larvae acclimated to 20 psu showed better growth performance and body energetic content than post-larvae maintained at 38 psu. prl, adcyap1, and igf1 mRNA expression levels increased in 20-psu-acclimated post-larvae but decreased upon transfer to 38 psu. GH1 expression did not show significant changes under both experimental conditions. Our results suggested an enhanced general performance for post-larvae in brackish water, supported by the actions of adcyap1, igf1, and prl.

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Acknowledgments

This study was partly supported by Grants AGL2007-61211/ACU (Ministerio de Educación y Ciencia and FEDER, Spain), Proyecto de Excelencia PO7-RNM-02843 (Junta de Andalucía) to J.M.M., and AQUAGENOMICS (CDS2007-00002) (Spanish Ministry of Economic Affairs and Competitiveness-MINECO and FEDER/ERDF) to M.Y. Authors would like to thank Dr. Asmaa Galal-Khallaf for the precious help during the conduction of the experiment. Also, authors would like to thank Mr. Manuel Arjonilla and Ms. Esmeralda Ramos-García for conducting the biomass analyses and to CUPIMAR for providing experimental animals. K.M.G. was funded by a full doctorate fellowship from the Egyptian Bureau in Madrid and the Egyptian Government.

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Authors and Affiliations

  1. Instituto de Ciencias Marinas de Andalucía, Consejo Superior Investigaciones Científicas (ICMAN-CSIC), Campus de Excelencia Internacional del Mar (CEIMAR), 11519, Puerto Real, Cádiz, Spain

    Khaled Mohammed-Geba, Manuel Yúfera & Gonzalo Martínez-Rodríguez

  2. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Campus de Excelencia Internacional del Mar (CEIMAR), 11519, Puerto Real, Cádiz, Spain

    Khaled Mohammed-Geba & Juan Miguel Mancera

  3. Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Sciences, Menoufia University, Menoufia, Egypt

    Khaled Mohammed-Geba

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  1. Khaled Mohammed-Geba
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  2. Manuel Yúfera
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  3. Gonzalo Martínez-Rodríguez
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Correspondence to Khaled Mohammed-Geba.

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Mohammed-Geba, K., Yúfera, M., Martínez-Rodríguez, G. et al. Molecular endocrine changes of Gh/Igf1 axis in gilthead sea bream (Sparus aurata L.) exposed to different environmental salinities during larvae to post-larvae stages. Fish Physiol Biochem 42, 1177–1186 (2016). https://doi.org/10.1007/s10695-016-0207-5

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