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Molecular performance of Prl and Gh/Igf1 axis in the Mediterranean meager, Argyrosomus regius, acclimated to different rearing salinities

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Abstract

Aquaculture industry in the Mediterranean region exhibits a growing interest for the Mediterranean meager Argyrosomus regius. Some preliminary works showed a good growth performance of the species in nearly isosmotic salinities. However, the patterns of alteration of prolactin (Prl) as well as growth hormone (Gh)/insulin growth factor-1 (Igf1) axis at the molecular level are not yet described in this species. Therefore, we cloned and sequenced partial cDNAs for pituitary prolactin (prl) and growth hormone (gh), hepatic insulin-like growth factor (igf1), and β-actin (actb). Expression patterns of these transcripts were tested in juveniles of A. regius acclimated to four different environmental salinities: (1) 5 ‰ (hyposmotic); (2) 12 ‰ (isosmotic); (3) 38 ‰ (hyperosmotic; seawater control); and (4) 55 ‰ (extremely hyperosmotic). All investigated transcripts shared high sequence identities with their counterparts in other perciformes. prl mRNA levels showed inverse pattern with increasing salinities. gh mRNA enhanced significantly in both 12 and 55 ‰ salinity groups in comparison with the control group, while igf1 showed its maximum expression levels under the nearly isosmotic environment. The results indicated clear sensitivity of prl, gh and igf1 to changes in environmental salinity, which can possibly control the euryhalinity capacity of this species.

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Acknowledgments

This work was carried out as a part of the Spanish-Egyptian joint project AP/039755/11(Development of molecular, physiological and immunological biomarkers for the detection of stress related to the Mediterranean meager aquaculture Argyrosomus regius) awarded from the Spanish agency of international cooperation (Agencia Española de Cooperación Internacional, AECID) and the Egyptian Academy of Scientific Research and Technology, to Juan Miguel Mancera (Universidad de Cádiz, Spain) and Khaled Mohammed-Geba (Menofia University, Egypt). The authors would like to express their thanks to Prof. Saber Abd El-Rahman Sakr, head of the Zoology Department, Faculty of Science, Menofia University, Egypt, for his sincere help during the development of the project´s strategies and bureaucratic work.

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Authors and Affiliations

  1. Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Egypt

    Khaled Mohammed-Geba & Asmaa Galal-Khallaf

  2. Department of Biomedicine, Biotechnology, and Public Health, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cadiz, 11519, Puerto Real, Cádiz, Spain

    Antonio Astola González & Rubén Ayala Suárez

  3. Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), 11519, Puerto Real, Cádiz, Spain

    Khaled Mohammed-Geba, Asmaa Galal-Khallaf, Juan Antonio Martos-Sitcha & Gonzalo Martínez-Rodríguez

  4. Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cadiz, 11519, Puerto Real, Cadiz, Spain

    Khaled Mohammed-Geba, Asmaa Galal-Khallaf & Juan Miguel Mancera

  5. Immunology and Parasitology Division, Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Egypt

    Hany Mohammed Ibrahim

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  1. Khaled Mohammed-Geba
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Correspondence to Khaled Mohammed-Geba.

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This paper follows the ZFIN Zebrafish Nomenclature Guidelines for gene and protein names and symbols (https://wiki.zfin.org/display/general/ZFIN+Zebrafish+Nomenclature+Guidelines).

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Mohammed-Geba, K., González, A.A., Suárez, R.A. et al. Molecular performance of Prl and Gh/Igf1 axis in the Mediterranean meager, Argyrosomus regius, acclimated to different rearing salinities. Fish Physiol Biochem 43, 203–216 (2017). https://doi.org/10.1007/s10695-016-0280-9

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