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Conservation of somatic tissue derived from collared peccaries (Pecari tajacu Linnaeus, 1758) using direct or solid-surface vitrification techniques

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Abstract

Cryopreservation of somatic tissue can be applied in biodiversity conservation, especially for wild species as collared peccary. We aimed to evaluate the effect of vitrification techniques of ear tissue of collared peccary [direct vitrification in cryovials (DVC) or solid-surface vitrification (SSV)] on the layers of epidermis and dermis by conventional histology and cell ability during the in vitro culture. Thus, both the vitrification methods were able to maintain normal patterns of the epidermis as the cornea and granular layers, furthermore the intercellular space and dermal–epidermal junction of the spinous layer when compared to fresh control. Nevertheless, DVC and SSV percentage of normality decreased in the morphological integrity of cytoplasm (37.5 and 25.0%) of spinous layer, respectively, as compared to the fresh fragments (100%, p < 0.05). Moreover, other differences between the fresh control (100%) and DVC tissues were verified in the intra-epidermal cleavage of the spinous (37.5%) and basal (37.5%) layers. In general, DVC and SSV techniques were efficient for the recovery of the somatic cells according to most of the evaluated parameters for the in vitro culture (p > 0.05). In addition, only at time of 72 h (D3), in the growth curve, DVC fragments showed a reduced cell concentration than fresh control. In conclusion, SSV was found to be a more efficient method for vitrifying collared peccary skin tissue when compared to DVC. These results are relevant for the tissue cryopreservation from collared peccary and could also be useful for mammals with phylogenetic relationships.

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Acknowledgements

This study was supported by Brazilian Council of Scientific Development–CNPq (Process No. 477710/2013-1). The authors thank the Centre for Wild Animals Multiplication (CEMAS/UFERSA) for providing the animals and the Laboratory Gonadal Transplantation and In Vitro Embryo Production (LTG-PIV/UFERSA) for technical assistance. AR Silva and MF Oliveira were recipients of CNPq Grants.

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Authors and Affiliations

  1. Laboratory of Animal Biotechnology, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil

    Alana Azevedo Borges, Luiza Bento de Queiroz Neta, Maria Valéria de Oliveira Santos & Alexsandra Fernandes Pereira

  2. Laboratory of Applied Animal Morphophysiology, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil

    Moacir Franco de Oliveira

  3. Laboratory of Animal Germplasm Conservation, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil

    Gabriela Liberalino Lima & Alexandre Rodrigues Silva

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  1. Alana Azevedo Borges
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  2. Gabriela Liberalino Lima
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  4. Maria Valéria de Oliveira Santos
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Correspondence to Alexsandra Fernandes Pereira.

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Borges, A.A., Lima, G.L., de Queiroz Neta, L.B. et al. Conservation of somatic tissue derived from collared peccaries (Pecari tajacu Linnaeus, 1758) using direct or solid-surface vitrification techniques. Cytotechnology 69, 643–654 (2017). https://doi.org/10.1007/s10616-017-0074-7

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