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The use of a metal container for vitrification of mouse ovaries, as a clinical grade model for human ovarian tissue cryopreservation, after different times and temperatures of transport

  • Fertility Preservation
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Cryopreservation of ovarian tissue is paramount for fertility preservation, with important clinical applications, especially for women suffering from an oncological condition. Several cryopreservation methodologies have been tried in search of better outcomes, especially in terms of primor-dial and primary follicles integrity post-cryopreservation. Vitrification has successfully been applied to ovarian tissue using different carriers for tissue exposure to the liquid nitrogen (LN2).

Methods

We developed an enclosed metal vessel, which has the advantage of a faster heat transfer, when in contact with LN2 avoiding at the same time, the direct contact with tissue. Additionally, we assessed the effect of different times and temperatures of transport between the collection of mouse ovaries and the beginning of cryopreservation, on follicular morphology after vitrification.

Results

Our results suggest that 37 °C and R.T. help to maintain normal primordial and primary follicle morphology for up to 4 hrs after collection and beginning of vitrification in a metal container.

Conclusion

These data show that the metal container is an appropriate carrier for mouse ovary vitrification. The rate of morphologically normal primordial follicles up to 4 hrs.

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Acknowledgements

The authors are most thankful to Dr. Elsa Cristina de Mundstock from NAE-Federal University of Rio Grande do Sul, for the critical statistics help and supervision.

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Authors and Affiliations

  1. Department of Morphological Sciences, ICBS, Federal University of Rio Grande do Sul, CEP: 90.050-170, Porto Alegre, RS, Brazil

    Adriana Bos-Mikich & Nívia Lothhammer

  2. Reproduction Biotechnologies Laboratory, Veterinay School, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Lis Marques & José Luiz Rodrigues

  3. Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil

    Nilo Frantz

Authors
  1. Adriana Bos-Mikich
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  2. Lis Marques
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  3. José Luiz Rodrigues
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  4. Nívia Lothhammer
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  5. Nilo Frantz
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Corresponding author

Correspondence to Adriana Bos-Mikich.

Additional information

Capsule Vitrification of ovarian tissue in a metal container results in high rates of morphologically normal primordial follicles up to 4 hrs between tissue collection and cryopreservation.

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Bos-Mikich, A., Marques, L., Rodrigues, J.L. et al. The use of a metal container for vitrification of mouse ovaries, as a clinical grade model for human ovarian tissue cryopreservation, after different times and temperatures of transport. J Assist Reprod Genet 29, 1267–1271 (2012). https://doi.org/10.1007/s10815-012-9867-y

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  • DOI: https://doi.org/10.1007/s10815-012-9867-y

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