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Modulation of NaCl induced DNA damage and oxidative stress in mungbean by pretreatment with sublethal dose

  • Original Papers
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Biologia Plantarum

Abstract

Salinity is one of the most severe problems in worldwide agricultural production. The effect of salt on dry mass, total glutathione content, its regulatory enzymes, and extent of DNA damage in growing mungbean (Vigna radiata L. Wilczek) seedlings was investigated. The salt stress decreased a dry mass accumulation in the seedlings. A total glutathione (GSH) content and the activities of the enzymes of GSH metabolism were adversely affected by the salt stress. The enhanced accumulation of reactive oxygen species under the NaCl stress caused an increase in DNA damage, measured using a comet assay, in both roots and leaves of the mungbean seedlings. The pretreatment of mungbean seeds with a sublethal dose of NaCl was able to overcome the adverse effects of the salt stress to variable extents by exhibiting significant alterations of all tested parameters, imparting better growth and metabolism of the mungbean seedlings.

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Abbreviations

CDNB:

1-chloro-2,4-dinitrobenzene

Chl:

chlorophyll

DTNB:

5,5′-dithiobis-(2-nitrobenzoic acid)

EDTA:

ethylene diaminetetraacetic acid

GPx:

glutathione peroxidase

GR:

glutathione reductase

GSH:

glutathione (reduced)

GSSG:

glutathione (oxidized)

GST:

glutathione-S-transferase

MDA:

malondialdehyde

PMSF:

phenylmethane-sulphonyl fluoride

ROS:

reactive oxygen species

SSA:

sulphosalicylic acid

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Authors and Affiliations

  1. Plant Physiology and Biochemistry Laboratory, Centre of Advanced Study, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India

    P. Saha & A. K. Biswas

  2. Cell Biology and Genetic Toxicology Laboratory, Centre of Advanced Study, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India

    A. Mukherjee

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  1. P. Saha
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Correspondence to A. K. Biswas.

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Acknowledgements: This study was supported financially by a Research Project grant from the University Grants Commission, New Delhi.

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Saha, P., Mukherjee, A. & Biswas, A.K. Modulation of NaCl induced DNA damage and oxidative stress in mungbean by pretreatment with sublethal dose. Biol Plant 59, 139–146 (2015). https://doi.org/10.1007/s10535-014-0460-3

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  • DOI: https://doi.org/10.1007/s10535-014-0460-3

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