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Photobacterium sanguinicancri sp. nov. isolated from marine animals

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Abstract

Six strains were isolated from the hemolymph of the spider crab Maja brachydactyla, captured in Spain, and one from a diseased blue mussel, Mytilus edulis. The 16S rRNA gene sequences showed close similarity to the recently described Photobacterium swingsii (98.1 %) and to a lesser degree to Photobacterium aquimaris (97.8 %). MLSA analyses showed a monophyletic group including P. swingsii that form a new subclade. All genomic analyses (Average Nucleotide Identity, Average Amino Acid Identity, and in silico DNA–DNA) clearly separate the strains analysed from P. swingsii with values below the thresholds to delimit a new species. The phenotypic, genotypic and genomic data presented here clearly place these strains as a coherent group within the genus Photobacterium, for which we propose the name Photobacterium sanguinicancri sp. nov. Strain CAIM 1827T (=CECT 7579T, =DSM 24670T) is proposed as the type strain of the species.

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References

  • Auch AF, von Jan M, Klenk H-P, Göker M (2010) Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2:117–134. doi:10.4056/sigs.531120

    Article  PubMed  PubMed Central  Google Scholar 

  • Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O (2008) The RAST Server: rapid annotations using subsystems technology. BMC Genom 9:75

    Article  Google Scholar 

  • Cano-Gómez A, Goulden EF, Owens L, Høj L (2010) Vibrio owensii sp. nov., isolated from cultured crustaceans in Australia. FEMS Microbiol Lett 302:175–181. doi:10.1111/j.1574-6968.2009.01850.x

    Article  PubMed  Google Scholar 

  • Chimetto L, Cleenwerck I, Thompson CC, Brocchi M, Willems A, De Vos P, Thompson FL (2010) Photobacterium jeanii sp. nov., isolated from corals and zoanthids. Int J Syst Evol Microbiol 60:2843–2848. doi:10.1099/ijs.0.019968-0

    Article  CAS  PubMed  Google Scholar 

  • Cohn F (1878) Verzameling van stukken betreffende het Geneeskundig Staatstoezicht in Nederland. 126–130

  • Deep K, Poddar A, Das SK (2014) Photobacterium panuliri sp. nov., an Alkalitolerant Marine Bacterium Isolated from Eggs of Spiny Lobster, Panulirus penicillatus from Andaman Sea. Curr Microbiol 69:660–668. doi:10.1007/s00284-014-0638-0

    Article  CAS  PubMed  Google Scholar 

  • Eggermont M, Tamanji A, Nevejan N, Bossier P, Sorgeloos P, Defoirdt T (2014) Stimulation of heterotrophic bacteria associated with wild-caught blue mussel (Mytilus edulis) adults results in mass mortality. Aquaculture 431:136–138. doi:10.1016/j.aquaculture.2014.01.014

    Article  Google Scholar 

  • Gomez-Gil B, Roque A, Lacuesta B, Rotllant G (2010) Diversity of vibrios in the haemolymph of the spider crab Maja brachydactyla. J Appl Microbiol 109(3):918–926. doi:10.1111/j.1365-2672.2010.04718.x

    Article  CAS  PubMed  Google Scholar 

  • Gomez-Gil B, Thompson CC, Matsumura Y, Sawabe T, Iida T, Christen R, Thompson FL, Sawabe T (2014) The family Vibrionaceae. In: Rosenberg E (ed) The prokaryotes. Springer, Berlin, pp 659–747

    Google Scholar 

  • González-Castillo A, Enciso-Ibarrra J, Bolán-Mejia MC, Balboa S, Lasa A, Romalde JL, Cabanillas-Beltrán H, Gomez-Gil B (2015) Vibrio mexicanus sp. nov., isolated from a cultured oyster Crassostrea corteziensis. Antonie Van Leeuwenhoek 108:355–364

    Article  PubMed  Google Scholar 

  • Hasegawa M, Kishino H, Yano T (1985) Dating the human-ape split by a molecular clock of mitochondrial DNA. Evolution 22:160–174

    Article  CAS  Google Scholar 

  • Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23:254–267. doi:10.1093/molbev/msj030

    Article  CAS  PubMed  Google Scholar 

  • Kämpfer P, Kroppenstedt MR (1996) Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005. doi:10.1139/m96-128

    Article  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16s rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Krebs JE, Gale AN, Sontag TC, Keyser VK, Peluso EM, Newman JD (2013) A web-based method to calculate average amino acid identity (AAI) between prokaryotic genomes. Biotechniques

  • Lee I, Kim YO, Park S-C, Chun J (2015) OrthoANI: An improved algorithm and software for calculating average nucleotide identity. Int J Syst Bacteriol. In press

  • Lucena T, Ruvira MA, Pascual J, Garay E, Macián MC, Arahal DR, Pujalte MJ (2011) Photobacterium aphoticum sp. nov., isolated from coastal water. Int J Syst Evol Microbiol 61:1579–1584

    Article  CAS  PubMed  Google Scholar 

  • Macián MC, Ludwig W, Aznar R, Grimont PAD, Schleifer KH, Garay E, Pujalte MJ (2001) Vibrio lentus sp. nov., isolated from Mediterranean oysters. Int J Syst Evol Microbiol 51:1449–1456. doi:10.1099/00207713-51-4-1449

    Article  PubMed  Google Scholar 

  • Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60. doi:10.1186/1471-2105-14-60

    Article  Google Scholar 

  • Pascual J, Macián MC, Arahal DR, Garay E, Pujalte MJ (2010) Multilocus sequence analysis of the central clade of the genus Vibrio by using the 16S rRNA, recA, pyrH, rpoD, gyrB, rctB and toxR genes. Int J Syst Evol Microbiol 60:154–165

    Article  CAS  PubMed  Google Scholar 

  • Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 106:19126–19131. doi:10.1073/pnas.0906412106

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC News 20:16

    Google Scholar 

  • Sawabe T, Kita-Tsukamoto K, Thompson FL (2007) Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189:7932–7936. doi:10.1128/JB.00693-07

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sawabe T, Ogura Y, Matsumura Y, Feng G, Amin AR, Mino S, Nakagawa S, Sawabe T, Kumar R, Fukui Y, Satomi M, Matsushima R, Thompson FL, Gomez-Gil B, Christen R, Maruyama F, Kurokawa K, Hayashi T (2013) Updating the Vibrio clades defined by multilocus sequence phylogeny: proposal of eight new clades, and the description of Vibrio tritonius sp. nov. Front Microbiol 4:1–14

    Article  Google Scholar 

  • Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kämpfer P, Maiden MCJ, Nesme X, Rosselló-Mora R, Swings J, Trüper HG, Vauterin L, Ward AC, Whitman WB (2002) Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047

    CAS  PubMed  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson FL, Iida T, Swings J (2004) Biodiversity of vibrios. Microbiol Mol Biol Rev 68:403–431. doi:10.1128/MMBR.68.3.403-431.2004

    Article  Google Scholar 

  • Thompson CC, Vicente ACP, Souza RC, Vasconcelos ATR, Vesth T, Alves N, Ussery DW, Iida T, Thompson FL (2009) Genomic taxonomy of Vibrios. BMC Evol Biol 9:258

    Article  PubMed  PubMed Central  Google Scholar 

  • Varghese NJ, Mukherjee S, Ivanova N, Konstantinidis KT, Mavrommatis K, Kyrpides NC, Pati A (2015) Microbial species delineation using whole genome sequences. Nucleic Acids Res 43:6761–6771

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the Ad Hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464. doi:10.1099/00207713-37-4-463

    Article  Google Scholar 

  • Yoshizawa S, Wada M, Kita-Tsukamoto K, Yokota A, Kogure K (2009) Photobacterium aquimaris sp. nov., a luminous marine bacterium isolated from seawater. Int J Syst Evol Microbiol 59:1438–1442. doi:10.1099/ijs.0.004309-0

    Article  CAS  PubMed  Google Scholar 

  • Yoshizawa S, Wada M, Yokota A, Kogure K (2010) Vibrio sagamiensis sp. nov., luminous marine bacteria isolated from sea water. J Gen Appl Microbiol 56:499–507

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was partially funded by CONACYT Project CB-2009-01 132328. Thanks to Carmen Bolán Mejía and Julissa Enciso Barrón for technical help.

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Authors and Affiliations

  1. A.C. Mazatlán Unit for Aquaculture and Environmental Management, CIAD, AP. 711, Mazatlán, Sinaloa, 82000, Mexico

    Bruno Gomez-Gil

  2. Institut de Reçerca i Tecnologia Agroalimentaries, Centre d’Aquicultura, Santcarles de la Rapita, Spain

    Ana Roque

  3. Institut de Ciències del Mar, CSIC, Passeig Marítim de la Barceloneta, 37, 08003, Barcelona, Spain

    Guiomar Rotllant

  4. Departamento de Microbiología y Parasitología, CIBUS, Universidad de Santiago de Compostela, Campus Sur s/n, 15782, Santiago de Compostela, Spain

    Jesus L. Romalde & Alejandra Doce

  5. Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Rozier 44, 9000, Ghent, Belgium

    Mieke Eggermont & Tom Defoirdt

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  1. Bruno Gomez-Gil
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  2. Ana Roque
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  3. Guiomar Rotllant
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Correspondence to Bruno Gomez-Gil.

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Gomez-Gil, B., Roque, A., Rotllant, G. et al. Photobacterium sanguinicancri sp. nov. isolated from marine animals. Antonie van Leeuwenhoek 109, 817–825 (2016). https://doi.org/10.1007/s10482-016-0681-x

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  • DOI: https://doi.org/10.1007/s10482-016-0681-x

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