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Photobacterium panuliri sp. nov., an Alkalitolerant Marine Bacterium Isolated from Eggs of Spiny Lobster, Panulirus penicillatus from Andaman Sea

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Abstract

A facultative anaerobe, alkalitolerant, gram-negative marine bacterium strain LBS5T, was isolated from eggs carried on the pleopods of female spiny lobster (Panulirus penicillatus) in Andaman Sea from a depth of 3.5 m. Heterotrophic growth was observed at 15–38 °C and pH 5.5–11. Optimum growth occurred at 28 °C and pH 7.5. It can grow in the presence of 0.5–7 % NaCl (w/v), and the optimal NaCl required for growth was 2–4 %. 16S rRNA gene sequence analysis revealed the strain LBS5T belongs to the genus Photobacterium and showed 99.6 % similarity with P. aquae AE6T, 98.2 % with P. aphoticum M46T, 97 % with P. rosenbergii CC1T, 96.9 % with P. lutimaris DF-42T, and 96.6 % with P. halotolerans MACL01T. The DNA–DNA similarities between strains LBS5T with other closely related strains were well below 70 %. The DNA G + C content was 50.52 (±0.9) mol%. The major fatty acids were C16:1w7c/w6c, C18:1w6c/w7c, C16:0, C15:0 iso, C16:0 10-methyl/17:1 iso w9c, C17:0 iso. Polar lipids included a phosphatidylglycerol, a diphosphatidylglycerol, a phosphatidylethanolamine, and one unidentified lipid. Based on the polyphasic evidences, strain LBS5T represents a novel species of the genus Photobacterium for which Photobacterium panuliri sp. nov. is proposed. The type strain is LBS5T (=DSM 27646T = LMG 27617T = JCM 19199T).

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Acknowledgments

We are thankful to Prof. J. P. Euzéby (Ecole Nationale Vétérinaire, France) for etymological advice. We are grateful to Ministry of Environment and Forest, Wild life Division, Government of India and The Chief Conservator of Forests (Wild life), Andaman and Nicobar Islands, Port Blair, for officially allowing us to collect samples from the Andaman Sea. This work was supported in part by the funding received from Ministry of Earth Sciences, Government of India (MoES/11-MRDF/1/59/P/08) to SKD and Department of Biotechnology, Government of India. The authors, KD and AP acknowledge the University Grant Commission (UGC), and Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi, respectively, for providing the research fellowship.

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  1. Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751 023, India

    Kamal Deep, Abhijit Poddar & Subrata K. Das

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  1. Kamal Deep
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Correspondence to Subrata K. Das.

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Deep, K., Poddar, A. & Das, S.K. Photobacterium panuliri sp. nov., an Alkalitolerant Marine Bacterium Isolated from Eggs of Spiny Lobster, Panulirus penicillatus from Andaman Sea. Curr Microbiol 69, 660–668 (2014). https://doi.org/10.1007/s00284-014-0638-0

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