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Photobacterium lucens sp. nov., Isolated from a Cultured Shrimp Penaeus vannamei.

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Abstract

Two bacterial strains were isolated from the hepatopancreas of a cultured shrimp (Penaeus vannamei) in Sinaloa, México. Their partial 16S rRNA gene sequences clustered within those of the genus Photobacterium, showing high similarity to the type strains of Photobacterium angustum and Photobacterium leiognathi, were 87.1% and 97.5%, respectively. Multilocus sequence analysis using eight housekeeping genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA, topA and 16S rRNA) and phylogenetic analysis with 139 single-copy genes showed that the new strains form an independent branch whole genome sequencing and genomic analyses (average nucleotide identity, average amino acid identity, and in silico DNA–DNA hybridization) produced values well below the thresholds for species delineation with all methods tested. In addition, a phenotypic characterization was performed to support the description and differentiation of the novel strains from related taxa. The results obtained demonstrate that the two strains represent a novel species for which the name Photobacterium lucens sp. nov. is proposed.

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Acknowledgements

This study was funded by Centro de Investigación en Alimentación y Desarrollo.

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Authors and Affiliations

  1. CIAD, A.C., Mazatlán Unit for Aquaculture, AP. 711, 82000, Mazatlán, Sinaloa, México

    Julissa Enciso-Ibarra, Sonia A. Soto-Rodriguez, Karen Enciso-Ibarra, Carmen Bolán-Mejia & Bruno Gomez-Gil

  2. Unidad Académica de Ingeniería en Biotecnología, Universidad Politécnica de Sinaloa, Carretera Municipal Libre Km.3 Mazatlán-Higueras, 82199, Mazatlán, Sinaloa, Mexico

    Adrián González-Castillo

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  1. Julissa Enciso-Ibarra
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  2. Adrián González-Castillo
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  3. Sonia A. Soto-Rodriguez
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  4. Karen Enciso-Ibarra
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  5. Carmen Bolán-Mejia
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Correspondence to Bruno Gomez-Gil.

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The GenBank/EMBL/DDBJ accession number for genome and the Digital Protologue Database Taxon Number for strain are SSMG00000000 and TA01029, respectively.

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Enciso-Ibarra, J., González-Castillo, A., Soto-Rodriguez, S.A. et al. Photobacterium lucens sp. nov., Isolated from a Cultured Shrimp Penaeus vannamei.. Curr Microbiol 77, 1111–1116 (2020). https://doi.org/10.1007/s00284-020-01893-9

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  • DOI: https://doi.org/10.1007/s00284-020-01893-9

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