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Two-phase/Three-phase Hollow Fibre Liquid-Phase Simultaneous Microextraction Combined with HPLC for Analysis of Phenolic Acids and Flavonoids in Traditional Chinese Medicine

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Abstract

A two-phase/three-phase hollow fibre liquid-phase simultaneous microextraction (2p/3p-HF-LPSME) method, coupled with high-performance liquid chromatography and ultraviolet detection, was developed and introduced for simultaneous extraction and determination of phenolic acids and flavonoids in Lonicera japonica, Herba Taraxaci and Cortex Eucommiae. Several factors affecting performance were investigated and optimized, including the type of hollow fibre liquid-phase microextraction, extraction solvent, pHs of the sample and acceptor phases, extraction time, stirring rate, salt concentration in the sample solution and volume of sample phase. Under optimised conditions, the enrichment factors of 2p/3p-HF-LPSME for analytes ranged from 9 to 171, and good linearities were obtained for all analytes with regression coefficients of between 0.9939 and 0.9996. In addition, the limits of detection were between 0.3 and 4.0 ng·mL−1, and satisfactory recoveries (90.0–106.3 %) and precisions (RSD 2.3–10.4 %) were also achieved. The simultaneous microextraction mechanism of the approach was also analysed and described. Experimental results show that the method is simple, sensitive, practical and effective, and it can be used for simultaneous preconcentration and determination of phenolic acids and flavonoids in traditional Chinese medicines.

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Acknowledgments

This study was supported by the National Nature Science Foundation of China (No. 81302734), the Nature Science Foundation of Shanxi Province, China (No. 2012011007-4), and the Innovation Foundation of Shanxi Medical University (No. C01201010).

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Authors and Affiliations

  1. School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, China

    Shang Li, Shuang Hu, Xuan Chen, Xiaohong Bai & Shuqiu Zhang

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  1. Shang Li
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  2. Shuang Hu
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  3. Xuan Chen
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  4. Xiaohong Bai
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  5. Shuqiu Zhang
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Correspondence to Xiaohong Bai or Shuqiu Zhang.

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Li, S., Hu, S., Chen, X. et al. Two-phase/Three-phase Hollow Fibre Liquid-Phase Simultaneous Microextraction Combined with HPLC for Analysis of Phenolic Acids and Flavonoids in Traditional Chinese Medicine. Chromatographia 78, 1159–1167 (2015). https://doi.org/10.1007/s10337-015-2930-y

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  • DOI: https://doi.org/10.1007/s10337-015-2930-y

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