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Rapid Change of Microbiota Diversity in the Gut but Not the Hepatopancreas During Gonadal Development of the New Shrimp Model Neocaridina denticulata

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Abstract

During evolution of animals, their co-evolution with bacteria has generally been ignored. Recent studies have provided evidences that the symbiotic bacteria in the animal gut can either be essential or contributing to the plasticity of the host. The Crustacea includes crab, crayfish, lobster, and shrimp and represents the second largest subphylum on the planet. Although there are already studies investigating the intestinal bacterial communities in crustaceans, none of them has examined the microbiota in different parts of the digestive system during the gonad development of the host. Here, we utilized a new shrimp model Neocaridina denticulata and sequenced the 16S rRNA using the Ion Torrent platform to survey the bacterial populations colonizing the hepatopancreas, foregut, and intestine, including midgut and hindgut, of the early, mid, and late ovarian maturation stages of the shrimp. The predominant bacteria phylum was found to be Proteobacteria, with more than 80 % reads from the gut flora at the early gonad development belonged to a Coxiella-type bacterium. Distinct bacterial communities can be detected between the hepatopancreas and gut, although no significant difference could be revealed between the different regions of the gut investigated. Surprisingly, during the gonad development, bacterial diversity changed rapidly in the gut but not the hepatopancreas. This study provides the first evidence that microbiota modified differentially in specific regions of the digestive tract during gonadal development of crustaceans.

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Acknowledgments

This work was supported by a CRF grant (C4024-14G) from the Research Grants Council, Hong Kong Special Administrative Region, China, and grants from The Chinese University of Hong Kong.

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Author notes

  1. Patrick Tik Wan Law

    Present address: The Nethersole School of Nursing, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR

Authors and Affiliations

  1. School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR

    Man Kit Cheung, Patrick Tik Wan Law & Hoi Shan Kwan

  2. Simon F.S. Li Marine Science Laboratory, School of Life Sciences, Centre for Soybean Research, Partner State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR

    Ho Yin Yip, Wenyan Nong & Jerome Ho Lam Hui

  3. Simon F.S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR

    Ka Hou Chu

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Correspondence to Jerome Ho Lam Hui.

Additional information

Data Deposition

Data has been deposited in NCBI Sequence Read Archive (Accession number SRR1735538).

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Figure S1

PCoA plots built using unweighted UniFrac distances. In the left panel, samples were colored according to the sampling position; in the right panel, samples were colored according to the ovarian maturation stage. (PPTX 347 kb)

Figure S2

PCoA plots built using weighted UniFrac distances. In the left panel, samples were colored according to the sampling position; in the right panel, samples were colored according to the ovarian maturation stage. (PPTX 394 kb)

Figure S3

Relative abundance of major bacterial genera recovered in the gut and hepatopancreas samples. Only genera with >1 % average relative abundance were shown here. See Table 1 for abbreviations of samples. (PPTX 104 kb)

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Cheung, M.K., Yip, H.Y., Nong, W. et al. Rapid Change of Microbiota Diversity in the Gut but Not the Hepatopancreas During Gonadal Development of the New Shrimp Model Neocaridina denticulata . Mar Biotechnol 17, 811–819 (2015). https://doi.org/10.1007/s10126-015-9662-8

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