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LecRK-VII.1, a Lectin Receptor-Like Kinase, Mediates the Regulation of Salt Stress and Jasmonic Acid Response in Arabidopsis

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Abstract

Lectin receptor-like kinases (LecRKs) are a class of membrane proteins found in higher plants. They play important roles in defense against of pathogens, perception of insect feeding, and stress tolerance. LecRK-VII.1 (AT4G04960) is one member of the LecRK family, which contains a conserved lectin domain and a kinase domain. In this study, the lecrk-VII.1 mutants (lecrk-VII.1-1 and lecrk-VII.1-2) showed increased seed germination rates and primary root length compared to ecotype Columbia-0 (Col-0) when treated with different concentrations of NaCl and methyl jasmonate (MeJA). To further confirm the possible role of LecRK-VII.1 in salt and jasmonic acid (JA) responses, we performed a comparative proteomic analysis of Arabidopsis Col-0 and lecrk-VII.1-1 mutant seedlings. In total, 312 proteins were differentially expressed of which 75 were related to stress responses including salt stress and JA response. Among them, decreased expressions of lipoxygenase (LOX) and annexin were confirmed by RT-PCR analyses. Activity of LOX was accordingly reduced in lecrk-VII.1 mutants. These results provide some valuable data to understand the role of LecRK-VII.1 in the response to salt stress and MeJA.

Graphical Abstract

In order to understand the role of LecRK-VII.1 in the response to salt stress and MeJA, we studied the phenotypes and expression patterns between the lecrk-VII.1 mutants and wild type. Comparative proteomic analysis of differentially expressed proteins of the lecrk-VII.1 mutants and wild type in Arabidopsis was performed.

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Acknowledgements

This research was supported by Grants from the National Key Laboratory of Plant Molecular Genetics (2015), the National Natural Science Foundation of China (31540064, 31071076, and 30871325), the PhD Programs Foundation of Ministry of Education of China (20130161110005), the Key Scientific and Technological Project of Hunan Province-International and Regional Science and Technology Innovation Cooperation (2016WK2003), Hunan Provincial Innovation Foundation for Postgraduate (CX2015B073, CX2016B097), and Changsha Science and Technology Funds (K1406036-21).

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    1. College of Biology, Hunan University, Changsha, 410082, People’s Republic of China

      Cheng Zhang, Jinyan Li, Xinhong Guo, Wenjun Xiao, Ping Wang, Shuai Hu, Xiutao Lu & Zhuang He

    2. Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, 410081, People’s Republic of China

      Baode Zhu, Miao Jiang & Ping Chen

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    1. Cheng Zhang
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    Correspondence to Xinhong Guo or Ping Chen.

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    Cheng Zhang and Jinyan Li have contributed equally to this work.

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    344_2016_9647_MOESM1_ESM.tif

    Supplementary material 1 (TIFF 40 kb). Fig. S1. Immunoblots of total protein and crude membrane protein of Col-0. Total protein and crude membrane proteins were separated by SDS-PAGE. Western blots were probed using membrane marker protein H+–ATP antibodies

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    Zhang, C., Li, J., Guo, X. et al. LecRK-VII.1, a Lectin Receptor-Like Kinase, Mediates the Regulation of Salt Stress and Jasmonic Acid Response in Arabidopsis . J Plant Growth Regul 36, 385–401 (2017). https://doi.org/10.1007/s00344-016-9647-5

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