Abstract
Lectin receptor-like kinases (LecRK) are widespread in higher plants; however, little is known about their physiological roles. In this study, At1g70130 (designated LecRK-b2), an Arabidopsis LecRK gene, has been investigated. LecRK-b2 was predominantly expressed during seed germination, and its expression was ceased following germination. The expression of LecRK-b2 was induced by abscisic acid (ABA), salt, and osmotic stress. LecRK-b2 loss-of-function mutation slightly reduced the ABA sensitivity during seed germination, and this reduced sensitivity was demonstrated not due to lower ABA accumulation level in the seeds. Dual-luciferase transient expression assay confirmed that the transcription factor ABSCISIC ACID INSENSITIVE3 (ABI3) could activate the luciferase under driving of LecRK-b2 promoter. LecRK-b2 transcription level was found to be down-regulated in abi3 during seed germination. Furthermore, LecRK-b2 loss-of-function mutation reduced the salt and osmotic sensitivity during early development stage of Arabidopsis. Taken together, these results suggest that LecRK-b2 functions as a positive regulator of the ABA response during the seed germination and is involved in salt and osmotic stress response in the early development stage.
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Acknowledgment
This research was supported by grants from the National Natural Science Foundation of China (30600368, 30770200, and 30871325).
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Fig. S1
Putative cis elements involved in the regulation of the LecRK-b2 gene. (A and B) RY/G-like element, (C) start codon, (D and E) ACGT-core motif. (DOC 395 kb)
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Deng, K., Wang, Q., Zeng, J. et al. A Lectin Receptor Kinase Positively Regulates ABA Response During Seed Germination and Is Involved in Salt and Osmotic Stress Response. J. Plant Biol. 52, 493–500 (2009). https://doi.org/10.1007/s12374-009-9063-5
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DOI: https://doi.org/10.1007/s12374-009-9063-5