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Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols

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Abstract

Plant transformation protocols generally involve the use of selectable marker genes for the screening of transgenic material. The bacterial gene nptII, coding for a neomycin phosphotransferase, and the hpt gene, coding for a hygromycin phosphotransferase, are frequently used. These enzymes detoxify aminoglycoside antibiotics by phosphorylation, thereby permitting cell growth in the presence of antibiotics. Nevertheless, the screening for transgenic regenerated shoots is often partial and difficult due to regeneration of escapes and chimeras. These difficulties can be caused, in part, by an incorrect assumption about the mode of action of antibiotics in bacterial and eukaryotic cells and in in vitro tissue culture. The information contained in this review could be useful to establish better selection strategies by taking into account factors such as explant complexity, transformation and selection protocols that allow better accessibility to cells of Agrobacterium and antibiotics, and faster regeneration methods that avoid collateral effects of antibiotics on recovered, putative transgenic shoots.

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Acknowledgments

This work was partially supported by BIOCARM BIO-AGR07/04-0011 and Séneca 08665/PI/08. IMGP thanks the Spanish Council of Scientific Research (CSIC) for a I3P post-doctoral contract. The authors thank Prof. Fernando Pliego-Alfaro for the critical review of the manuscript and Dr. David J. Walker for English language editing.

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  1. I. M. G. Padilla

    Present address: IFAPA Centro de Churriana, 29410, Malaga, Spain

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  1. Grupo de Biotecnología de Frutales, Departamento de Mejora, CEBAS-CSIC, Campus Universitario de Espinardo, Apartado de correos 164, 30100, Murcia, Spain

    I. M. G. Padilla & L. Burgos

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  1. I. M. G. Padilla
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  2. L. Burgos
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Correspondence to I. M. G. Padilla.

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Communicated by R. Reski.

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Padilla, I.M.G., Burgos, L. Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols. Plant Cell Rep 29, 1203–1213 (2010). https://doi.org/10.1007/s00299-010-0900-2

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  • DOI: https://doi.org/10.1007/s00299-010-0900-2

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