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Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera)

  • Genetic Transformation and Hybridization
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Abstract

The pea (Pisum sativum L.) varieties Baroness (United Kingdome) and Baccara (France) were transformed via Agrobacterium tumefaciens-mediated gene transfer with pGPTV binary vectors containing the bar gene in combination with two different antifungal genes coding for polygalacturonase-inhibiting protein (PGIP) from raspberry (Rubus idaeus L.) driven by a double 35S promoter, or the stilbene synthase (Vst1) from grape (Vitis vinifera L.) driven by its own elicitor-inducible promoter. Transgenic lines were established and transgenes combined via conventional crossing. Resveratrol, produced by Vst1 transgenic plants, was detected using HPLC and the PGIP expression was determined in functional inhibition assays against fungal polygalacturonases. Stable inheritance of the antifungal genes in the transgenic plants was demonstrated.

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Abbreviations

FW:

Fresh weight

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcriptase PCR

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Acknowledgement

We express our gratitude to Dr. D. Berger, University of Pretoria, South Africa for providing the fungus, Dr Brian Williamson and Dr Craig Simpson, SCRI Scotland, for the raspberry PGIP gene and Geetha Senthil, SCRI, for the PGIP-containing constructs. Last but not least we thank Günter Stoschek, Daniela Pontiggia, Maja Marheine and Claudia Wichmann.

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Authors and Affiliations

  1. Department of Molecular Genetics, University of Hannover, Herrenhäuserstr 2, 30419, Hannover, Germany

    A. Richter & H.-J. Jacobsen

  2. de Kathen & Pickardt BioTechConsult GbR, Pestalozzi Strasse 35, 10627, Berlin, Germany

    A. de Kathen

  3. Dip. Biologia Vegetale Università “La Sapienza”, Piazza A. Moro 5, 00185, Roma, Italy

    G. de Lorenzo

  4. Institute for Nutritional Physiology, Federal Research Center for Nutrition, Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany

    K. Briviba

  5. Bayer CropScience GmBH, Global Biology Herbicides Biochemistry, 65926, Frankfurt-am-Main, Germany

    R. Hain

  6. Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

    G. Ramsay

  7. German Collection of Microorganisms and Cell Cultures GmbH, Mascheroder Weg 1b, 38124, Braunschweig, Germany

    H. Kiesecker

Authors
  1. A. Richter
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  2. H.-J. Jacobsen
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  3. A. de Kathen
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  4. G. de Lorenzo
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  5. K. Briviba
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  6. R. Hain
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  7. G. Ramsay
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  8. H. Kiesecker
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Corresponding author

Correspondence to H. Kiesecker.

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Communicated by H. Lörz

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Richter, A., Jacobsen, HJ., de Kathen, A. et al. Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera). Plant Cell Rep 25, 1166–1173 (2006). https://doi.org/10.1007/s00299-006-0172-z

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  • DOI: https://doi.org/10.1007/s00299-006-0172-z

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