Abstract
A reliable Agrobacterium tumefaciens-mediated transformation method has been developed for peas (Pisum sativum) using immature cotyledons as the explant source. Transgenic plants were recovered from the four cultivars tested: Bolero, Trounce, Bohatyr and Huka. The method takes approximately 7 months from explant to seed-bearing primary regenerant. The binary vector used carried genes for kanamycin and phosphinothricin resistance. Transformed pea plants were selected on 10 mg/l phosphinothricin. The nptII and bar genes were shown to be stably inherited through the first sexual generation of transformed plants. Expression of the phosphinothricin-resistance gene in the transformed plants was demonstrated using the ‘Buster’ (=‘Basta’) leaf-paint test and the phosphinothricin acetyl transferase enzyme assay.
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Abbreviations
- BA:
-
6-benzylaminopurine
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Communicated by E. D. Earle
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Grant, J.E., Cooper, P.A., McAra, A.E. et al. Transformation of peas (Pisum sativum L.) using immature cotyledons. Plant Cell Reports 15, 254–258 (1995). https://doi.org/10.1007/BF00193730
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DOI: https://doi.org/10.1007/BF00193730