Abstract
Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and β-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.
This is a preview of subscription content, log in via an institution to check access.
References
Becker D: Binary vectors which allow the exchange of plant selectable markers and reporter genes. Nucl Acids Res 18: 203 (1990).
Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).
DeBlock M, Botterman J, Vandewiele M, Dockx J, Thoen C, Gossele V, Movva NR, Thompson C, VanMontagu M, Leemans J: Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J 6: 2513–2518 (1987).
Hille J, Verheggen F, Roelvink P, Franssen H, Kammen AV, Zabel P: Bleomycin resistance: a new dominant selectable marker for plant cell transformation. Plant Mol Biol 7: 171–176 (1986).
Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
Kemper E, Grevelding C, Schell J, Masterson R: Improved method for the transformation of Arabidopsis thaliana with chimeric dihydrofolate reductase constructs which confer methotrexate resistance. Plant Cell Rep 11: 118–121 (1992).
Töpfer R, Gronenborn B, Schell J, Steinbiss H-H: Uptake and transient expression of chimeric genes in seedderived embryos. Plant Cell 1: 133–139 (1980).
White J, Chang S-YP, Bibb MJ, Bibb MJ: A cassette containing the bar gene of S. hygroscopicus: a selectable marker for plant transformation. Nucl Acids Res 18: 1062 (1990).
Yenofsky RL, Fine M, Pellows JW: A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure. Proc Natl Acad Sci USA 87: 3435–3439 (1990).
Zambryski P: Basic processes underlying Agrobacterium-mediated DNA transfer to plant cells. Annu Rev Genet 22: 1–30 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Becker, D., Kemper, E., Schell, J. et al. New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol Biol 20, 1195–1197 (1992). https://doi.org/10.1007/BF00028908
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00028908