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Detailed mapping of a resistance locus against Fusarium wilt in cultivated eggplant (Solanum melongena)

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This is the first report on genetic mapping of a resistance locus against Fusarium wilt caused by the plant pathogen Fusarium oxysporum f. sp. melongenae in cultivated eggplant.

Abstract

Fusarium wilt, caused by the plant pathogen Fusarium oxysporum f. sp. melongenae, is a major soil-borne disease threatening stable production in eggplant (Solanum melongena). Although three eggplant germplasms, LS1934, LS174, and LS2436, are known to be highly resistant to the pathogen, their resistance loci have not been mapped. In this study, we performed quantitative trait locus analyses in F2:3 populations and detected a resistance locus, FM1, at the end of chromosome 2, with two alleles, Fm1 L and Fm1 E, in the F2 populations LWF2 [LS1934 × WCGR112-8 (susceptible)] and EWF2 [EPL-1 (derived from LS174) × WCGR112-8], respectively. The percentage of phenotypic variance explained by Fm1 L derived from LS1934 was 75.0 % [Logarithm of the odds (LOD) = 29.3], and that explained by Fm1 E derived from EPL-1 was 92.2 % (LOD = 65.8). Using backcrossed inbred lines, we mapped FM1 between two simple sequence repeat markers located ~4.881 cM apart from each other. Comparing the location of the above locus to those of previously reported ones, the resistance locus Rfo-sa1 from an eggplant ally (Solanum aethiopicum gr. Gilo) was mapped very close to FM1, whereas another resistance locus, from LS2436, was mapped to the middle of chromosome 4. This is the first report of mapping of a Fusarium resistance locus in cultivated eggplant. The availability of resistance-linked markers will enable the application of marker-assisted selection to overcome problems posed by self-incompatibility and introduction of negative traits because of linkage drag, and will lead to clear understanding of genetic mechanism of Fusarium resistance.

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References

  • Altınok HH (2005) First report of Fusarium wilt of eggplant caused by Fusarium oxysporum f. sp. melongenae in Turkey. New Disease Reports 11:5

  • Barchi L, Lanteri S, Portis E, Stagel A, Vale G, Toppino L, Rotino GL (2010) Segregation distortion and linkage analysis in eggplant (Solanum melongena L.). Genome 53:805–815

    Article  PubMed  CAS  Google Scholar 

  • Barchi L, Lanteri S, Portis E, Vale G, Volante A, Pulcini L, Ciriaci T, Acciarri N, Barbierato V, Toppino L, Rotino GL (2012) A RAD tag derived marker based eggplant linkage map and the location of QTLs determining anthocyanin pigmentation. PLoS One 7:e43740

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Bournival BL, Vallejos CE, Scott JW (1990) Genetic analysis of resistances to races 1 and 2 of Fusarium oxysporum f. sp. lycopersici from the wild tomato Lycopersicon pennellii. Theor Appl Genet 79:641–645

    Article  PubMed  CAS  Google Scholar 

  • Brotman Y, Kovalski I, Dogimont C, Pitrat M, Portnoy V, Perl-Treves R (2005) Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon. Theor Appl Genet 110:337–345

    Article  PubMed  CAS  Google Scholar 

  • Brotman Y, Normantovich M, Goldenberg Z, Zvirin Z, Kovalski I, Stovbun N, Donniger T, Bolger AM, Troadec C, Bendahmane A, Cohen R, Katzir N, Pitrat M, Dogimont C, Perl-Treves R (2012) Dual resistance of melon to Fusarium oxysporum races 0 and 2 and to Papaya ring-spot virus is controlled by a pair of head-to-head oriented NB-LRR genes of unusual architecture. Mol Plant 6(1):235–238

    Article  PubMed  Google Scholar 

  • Brown JKM (2002) Yield penalties of disease resistance in crops. Curr Opin Plant Biol 5:339–344

    Article  PubMed  CAS  Google Scholar 

  • Calvo-Asensio I, Prohens J, Gisbert C (2014) Vigor for in vitro culture traits in S. melongena × S. aethiopicum hybrids with potential as rootstocks for eggplant. Sci World. doi:10.1155/2014/702071

    Google Scholar 

  • Chittaranjan K (2011) Wild crop relatives: genomic and breeding resources - vegetables. Springer, Berlin, pp 1–282

    Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  PubMed Central  Google Scholar 

  • Daunay MC (2008) Eggplant. Handbook of plant breeding Vegetables II Fabaceae, Liliaceae, Solanaceae, and Umbelliferae, p 163–220

  • Daunay MC, Chaput MH, Sihachakr D, Allot M, Vedel F, Ducreux G (1993) Production and characterization of fertile somatic hybrids of eggplant (Solanum melongena L) with Solanum aethiopicum L. Theor Appl Genet 85:841–850

    PubMed  CAS  Google Scholar 

  • FAO (2004) FAOSTAT. Food and Agriculture Organization of the United Nations. http://faostat.fao.org

  • FAO (2013) FAOSTAT. Food and Agriculture Organization of the United Nations. http://faostat.fao.org

  • Fukuoka H, Miyatake K, Negoro S, Nunome T, Ohyama A, Yamaguchi H (2008) Development of a routine procedure for single nucleotide polymorphism marker design based on the Tm-shift genotyping method. Breed Sci 58:461–464

    Article  CAS  Google Scholar 

  • Fukuoka S, Saka N, Koga H, Ono K, Shimizu T, Ebana K, Hayashi N, Takahashi A, Hirochika H, Okuno K, Yano M (2009) Loss of function of a proline-containing protein confers durable disease resistance in rice. Science 325(5943):998–1001

    Article  PubMed  CAS  Google Scholar 

  • Fukuoka H, Miyatake K, Nunome T, Negoro S, Shirasawa K, Isobe S, Asamizu E, Yamaguchi H, Ohyama A (2012) Development of gene-based markers and construction of an integrated linkage map in eggplant by using Solanum orthologous (SOL) gene sets. Theor Appl Genet 125:47–56

    Article  PubMed  CAS  Google Scholar 

  • Haggard JE, Johnson EB, Clair DA (2013) Linkage relationships among multiple QTL for horticultural traits and late blight (P. infestans) resistance on chromosome 5 introgressed from wild tomato Solanum habrochaites. G3 3:2131–2146

    Article  PubMed  PubMed Central  Google Scholar 

  • Hemming MN, Basuki S, McGrath DJ, Carroll BJ, Jones DA (2004) Fine mapping of the tomato I-3 gene for fusarium wilt resistance and elimination of a co-segregating resistance gene analogue as a candidate for I-3. Theor Appl Genet 109:409–418

    Article  PubMed  CAS  Google Scholar 

  • Herman R, Zvirin Z, Kovalski I, Freeman S, Denisov Y, Zuri G, Katzir N, Perl-Treves R (2008) Characterization of Fusarium race 1,2 resistance in melon and mapping of a major QTL for this trait near a fruit netting locus. Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae, Avignon, France, pp 149–156

  • Hirakawa H, Shirasawa K, Miyatake K, Nunome T, Negoro S, Ohyama A, Tamaguchi H, Sato S, Isobe S, Tabata S, Fukuoka H (2014) Draft genome sequence of eggplant (Solanum melongena L.): the representative Solanum species indigenous to the Old World. DNA Res . doi:10.1093/dnares/dsu027 (in press)

    Google Scholar 

  • Joobeur T, King JJ, Nolin SJ, Thomas CE, Dean RA (2004) The Fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features. Plant J 39:283–297

    Article  PubMed  CAS  Google Scholar 

  • Kashyap V, Kumar SV, Collonnier C, Fusari F, Haicour R, Rotino GL, Sihachakr D, Rajam M (2003) Biotechnology of eggplant. Sci Hortic 97:1–25

    Article  CAS  Google Scholar 

  • Khan RS, Ntui VO, Chin DP, Nakamura I, Mii M (2011) Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker. Plant Cell Rep 30:587–597

    Article  PubMed  CAS  Google Scholar 

  • Kikuchi K, Honda I, Matsuo S, Fukuda M, Saito T (2008) Stability of fruit set of newly selected parthenocarpic eggplant lines. Sci Hortic 115:111–116

    Article  Google Scholar 

  • Lander E, Abrahamson J, Barlow A, Daly M, Lincoln S, Newburg L, Green P (1987) MAPMAKER: a computer package for constructing genetic-linkage maps. Cytogenet Cell Genet 46:642-642

    Google Scholar 

  • Legg PD, Litton CC, Collins GB (1981) Effects of the Nicotiana debneyi black root rot resistance factor on agronomic and chemical traits in burley tobacco. Theor Appl Genet 60:365–368

    Article  PubMed  CAS  Google Scholar 

  • Lehmann EL (1975) Nonparametrics. McGraw-Hill, New York

    Google Scholar 

  • Lewis RS, Linger LR, Wolff MF, Wernsman EA (2007) The negative influence of N-mediated TMV resistance on yield in tobacco: linkage drag versus pleiotropy. Theor Appl Genet 115:169–178

    Article  PubMed  CAS  Google Scholar 

  • Lim GT, Wang GP, Hemming MN, McGrath DJ, Jones DA (2008) High resolution genetic and physical mapping of the I-3 region of tomato chromosome 7 reveals almost continuous microsynteny with grape chromosome 12 but interspersed microsynteny with duplications on Arabidopsis chromosomes 1, 2 and 3. Theor Appl Genet 118:57–75

    Article  PubMed  CAS  Google Scholar 

  • Matsuo T, Ishigami K (1958) On the wilt of Solanum melongena L. and its casual fungus Fusarium oxysporum f. melongenae n.f. Phytopathol Soc Jpn 23:189–192 (in Japanese with English summary)

    Article  Google Scholar 

  • Miyatake K, Saito T, Negoro S, Yamaguchi H, Nunome T, Ohyama A, Fukuoka H (2012) Development of selective markers linked to a major QTL for parthenocarpy in eggplant (Solanum melongena L.). Theor Appl Genet 124:1403–1413

    Article  PubMed  Google Scholar 

  • Mochizuki H, Sakata Y, Yamakawa K, Nishio T, Komochi S, Narikawa T, Monma S (1997) ‘Eggplant parental line 1’, an eggplant breeding line resistant to Fusarium wilt. Bulletin of the National Institute of Vegetable and Tea Science 12:85–90 (in Japanese with English summary)

    Google Scholar 

  • Monma S, Sato T, Matsunaga H (1996) Evaluation of resistance to bacterial, fusarium and verticillium wilt in eggplant and eggplant related species collected in Ghana. J Jpn Soc Hortic Sci 65:278–279 (in Japanese with English summary)

    Google Scholar 

  • Monma S, Akazawa S, Simosaka K, Sakata Y, Matsunaga H (1997) ‘Daitaro’, a bacterial wilt- and Fusarium wilt-resistant hybrid eggplant for rootstock. Bull Natl Inst Veg Ornam Plants Tea 12:73–83 (in Japanese with English summary)

    Google Scholar 

  • Mutlu N, Boyaci FH, Gocmen M, Abak K (2008) Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant. Theor Appl Genet 117:1303–1312

    Article  PubMed  CAS  Google Scholar 

  • Nunome T, Ishiguro K, Yoshida T, Hirai M (2001) Mapping of fruit shape and color development traits in eggplant (Solanum melongena L.) based on RAPD and AFLP markers. Breed Sci 51:19–26

    Article  CAS  Google Scholar 

  • Nunome T, Suwabe K, Iketani H, Hirai M (2003a) Identification and characterization of microsatellites in eggplant. Plant Breed 122:256–262

    Article  CAS  Google Scholar 

  • Nunome T, Suwabe K, Ohyama A, Fukuoka H (2003b) Characterization of trinucleotide microsatellites in eggplant. Breed Sci 53:77–83

    Article  CAS  Google Scholar 

  • Nunome T, Negoro S, Kono I, Kanamori H, Miyatake K, Yamaguchi H, Ohyama A, Fukuoka H (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor Appl Genet 119:1143–1153

    Article  PubMed  Google Scholar 

  • Ori N, Eshed Y, Paran I, Presting G, Aviv D, Tanksley S, Zamir D, Fluhr R (1997) The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes. Plant Cell 9:521–532

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Oumouloud A, Arnedo-Andres MS, Gonzalez-Torres R, Alvarez JM (2008) Development of molecular markers linked to the Fom-1 locus for resistance to Fusarium race 2 in melon. Euphytica 164:347–356

    Article  CAS  Google Scholar 

  • Oumouloud A, Arnedo-Andres MS, Gonzalez-Torres R, Alvarez JM (2010) Inheritance of resistance to Fusarium oxysporum f. sp. melonis races 0 and 2 in melon accession Tortuga. Euphytica 176:183–189

    Article  Google Scholar 

  • Perchepied L, Dogimont C, Pitrat M (2005) Strain specific and QTL involved in the control of partial resistance to Fusarium oxysporum f. sp. melonis race 1,2 in a recombinant inbred line population of melon. Theor Appl Genet 111:65–74

    Article  PubMed  CAS  Google Scholar 

  • Portis E, Barchi L, Toppino L, Lanteri S, Acciarri N, Felicioni N, Fusari F, Barbierato V, Cericola F, Vale G, Rotino GL (2014) QTL mapping in eggplant reveals clusters of yield-related loci and orthology with the tomato genome. PLoS One 9:e89499

    Article  PubMed  PubMed Central  Google Scholar 

  • Rizza F, Mennella G, Collonnier C, Shiachakr D, Kashyap V, Rajam MV, Prestera M, Rotino GL (2002) Androgenic dihaploids from somatic hybrids between Solanum melongena and S-aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp melongenae. Plant Cell Rep 20:1022–1032

    Article  CAS  Google Scholar 

  • Safikhani N, Morid B, Zamanizadeh HR (2013) First report of Fusarium wilt of eggplant caused by Fusarium oxysporum f. sp. melongenae in Iran. New Disease Reports 28:16

  • Saito T, Matsunaga H, Saito A (2010) Development of Solanum melongena breeding lines as resistant rootstocks to Verticillium, bacterial, and Fusarium wilts. XIVth EUCARPIA Working Group Meeting on Genetics and Breeding of Capsicum and Eggplant Abstract book:513–520

  • Sakata Y, Monma S, Narikawa T, Komochi S (1996) Evaluation of resistance to bacterial wilt and Verticillium wilt in eggplants (Solanum melongena L) collected in Malaysia. J Jpn Soc Hortic Sci 65:81–88

    Article  Google Scholar 

  • Sarfatti M, Katan J, Fluhr R, Zamir D (1989) An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2. Theor Appl Genet 78:755–759

    Article  PubMed  CAS  Google Scholar 

  • Sarfatti M, Abu-Abied M, Katan J, Zamir D (1991) RFLP mapping of I1, a new locus in tomato conferring resistance against Fusarium oxysporum f. sp. lycopersici race 1. Theor Appl Genet 82:22–26

    Article  PubMed  CAS  Google Scholar 

  • Schaff DA, Jelenkovic G, Boyer CD, Pollack BL (1982) Hybridization and fertility of hybrid derivatives of Solanum melongena L and Solanum-macrocarpon L. Theor Appl Genet 62:149–153

    Article  PubMed  CAS  Google Scholar 

  • Segal G, Sarfatti M, Schaffer MA, Ori N, Zamir D, Fluhr R (1992) Correlation of genetic and physical structure in the region surrounding the I2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet 231:179–185

    PubMed  CAS  Google Scholar 

  • Simons G, Groenendijk J, Wijbrandi J, Reijans M, Groenen J, Diergaarde P, Van der Lee T, Bleeker M, Onstenk J, de Both M, Haring M, Mes J, Cornelissen B, Zabeau M, Vos P (1998) Dissection of the fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy. Plant Cell 10:1055–1068

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Steekelenburg N (1976) Fusarium wilt of eggplant in the Netherlands. Neth J Plant Pathol 82:191–192

    Article  Google Scholar 

  • Tezuka T, Waki K, Kuzuya M, Ishikawa T, Takatsu Y, Miyagi M (2011) Development of new DNA markers linked to the Fusarium wilt resistance locus Fom-1 in melon. Plant Breed 130:261–267

    Article  CAS  Google Scholar 

  • Toppino L, Vale G, Rotino GL (2008) Inheritance of Fusarium wilt resistance introgressed from Solanum aethiopicum Gilo and Aculeatum groups into cultivated eggplant (S. melongena) and development of associated PCR-based markers. Mol Breed 22:237–250

    Article  CAS  Google Scholar 

  • van Ooijen JW (2004) MapQTL® 6, software for the mapping of quantitative trait loci in experimental populations. Kyazma B. V., Wageningen

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  PubMed  CAS  Google Scholar 

  • Wang YH, Thomas CE, Dean RA (2000) Genetic mapping of a fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L.). Mol Breed 6:379–389

    Article  CAS  Google Scholar 

  • Wang S, Basten C, Zeng Z (2005) Windows QTL Cartographer 2.5. Department of Statistics North Carolina State University, Raleigh (http://statgen.ncsu.edu/qtlcart/WQTLCart.htm)

  • Yoshida T, Monma S, Matsunaga H, Sakata Y, Sato T, Saito T (2004) Development of a new rootstock eggplant cultivar ‘Daizaburou’ with high resistance to bacterial wilt and Fusarium wilt. Bull Natl Inst Veg Tea Sci 3:199–211 (in Japanese with English summary)

    Google Scholar 

  • Zeven AC, Knott DR, Johnson R (1983) Investigation of linkage drag in near isogenic lines of wheat by testing for seedling reaction to races of stem rust, leaf rust and yellow rust. Euphytica 32:319–327

    Article  Google Scholar 

  • Zink FW, Gubler WD (1985) Inheritance of resistance in muskmelon to Fusarium wilt. J Am Soc Hortic Sci 110(5):600–604

    Google Scholar 

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Acknowledgments

We are grateful to Ms. Toko Tani, Ms. Tomoe Shimamura, Ms. Yurika Kobayashi, and Ms. Naomi Fukushima for their technical assistance in NIVTS. This work was supported by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan (Development of mitigation and adaptation techniques to global warming in the sectors of agriculture, forestry, and fisheries (C-3-1030).

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  1. NARO Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Tsu, Mie, 514-2392, Japan

    Koji Miyatake, Takeo Saito, Satomi Negoro, Hirotaka Yamaguchi, Tsukasa Nunome, Akio Ohyama & Hiroyuki Fukuoka

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  1. Koji Miyatake
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  2. Takeo Saito
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  3. Satomi Negoro
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  4. Hirotaka Yamaguchi
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  7. Hiroyuki Fukuoka
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Correspondence to Koji Miyatake.

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Communicated by C. Gebhardt.

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122_2015_2632_MOESM2_ESM.pdf

Supplementary material 2 Position of the developed SNP marker, Sme2.5_14440_15, on the linkage map of LW2012 of chromosome 4 (Table 5) (PDF 46 kb)

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Supplementary material 4 (PDF 102 kb)

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Miyatake, K., Saito, T., Negoro, S. et al. Detailed mapping of a resistance locus against Fusarium wilt in cultivated eggplant (Solanum melongena). Theor Appl Genet 129, 357–367 (2016). https://doi.org/10.1007/s00122-015-2632-8

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