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Inheritance of Fusarium wilt resistance introgressed from Solanum aethiopicum Gilo and Aculeatum groups into cultivated eggplant (S. melongena) and development of associated PCR-based markers

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Abstract

The two eggplant relatives Solanum aethiopicum gr. Gilo and Solanum aethiopicum gr. Aculeatum (=Solanum integrifolium) carry resistance to the fungal wilt disease caused by Fusarium oxysporum f. sp. melongenae, a worldwide soil-borne disease of eggplant. To introgress the resistance trait into cultivated eggplant, the tetraploid somatic hybrids S. melongena + S. aethiopicum and S. melongena + S. integrifolium were used. An inheritance study of the resistance was performed on advanced anther culture-derived androgenetic backcross progenies from the two somatic hybrids. The segregation fitted a 3 resistant (R): 1 susceptible (S) ratio in the selfed populations and a 1R:1S ratio in the backcross progenies for the trait derived from S. aethiopicum and S. integrifolium. These ratios are consistent with a single gene, which we designated as Rfo-sa1, controlling the resistance to Fusarium oxysporum f. sp. melongenae. The allelic relationship between the resistance genes from S. aethiopicum and S. integrifolium indicate that these two genes are alleles of the same locus. Bulked Segregant Analysis (BSA) was performed with RAPD markers on the BC3/BC5 resistant advanced backcross progenies, and three RAPD markers associated with the resistance trait were identified. Cleaved Amplified Polymorphic Sequences (CAPSs) were subsequently obtained on the basis of the amplicon sequences. The evaluation of the efficiency of these markers in predicting the resistant phenotype in segregating progenies revealed that they represent useful tools for indirect selection of Fusarium resistance in eggplant.

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Acknowledgements

This research was partially supported by EU in the framework of the projects INCO-DC contract N° ICA4-CT-2001-10064 and by the Italian Ministry of Agricultural Alimentary and Forest Politics with funds released by C.I.P.E. (Resolution 17/2003) in the framework of the PROM project.

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Authors and Affiliations

  1. CRA-ORL Research Unit for Vegetable Crops, Via Paullese 28, 26836, Montanaso Lombardo, LO, Italy

    Laura Toppino & Giuseppe Leonardo Rotino

  2. CRA-GPG Genomic Research Center, Via S. Protaso 302, 29017, Fiorenzuola d’Arda, PC, Italy

    Giampiero Valè

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  1. Laura Toppino
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  2. Giampiero Valè
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  3. Giuseppe Leonardo Rotino
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Correspondence to Giuseppe Leonardo Rotino.

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Toppino, L., Valè, G. & Rotino, G.L. Inheritance of Fusarium wilt resistance introgressed from Solanum aethiopicum Gilo and Aculeatum groups into cultivated eggplant (S. melongena) and development of associated PCR-based markers. Mol Breeding 22, 237–250 (2008). https://doi.org/10.1007/s11032-008-9170-x

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