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The negative influence of N-mediated TMV resistance on yield in tobacco: linkage drag versus pleiotropy

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Abstract

Resistance to tobacco mosaic virus (TMV) is controlled by the single dominant gene N in Nicotiana glutinosa L. This gene has been transferred to cultivated tobacco (N. tabacum L.) by interspecific hybridization and backcrossing, but has historically been associated with reduced yields and/or quality in flue-cured tobacco breeding materials. Past researchers have suggested the role of pleiotropy and/or linkage drag effects in this unfavorable relationship. Introduction of the cloned N gene into a TMV-susceptible tobacco genotype (cultivar ‘K326’) via plant transformation permitted investigation of the relative importance of these possibilities. On average, yield and cash return ($ ha−1) of 14 transgenic NN lines of K326 were significantly higher relative to an isoline of K326 carrying N introduced via interspecific hybridization and backcrossing. The negative effects of tissue culture-induced genetic variation confounded comparisons with the TMV-susceptible cultivar, K326, however. Backcrossing the original transgenic lines to non-tissue cultured K326 removed many of these unfavorable effects, and significantly improved their performance for yield and cash return. Comparisons of the 14 corresponding transgenic NN backcross-derived lines with K326 indicated that linkage drag is the main factor contributing to reduced yields in TMV-resistant flue-cured tobacco germplasm. On average, these transgenic lines outyielded the conventionally-developed TMV-resistant K326 isoline by 427 kg ha−1 (P < 0.05) and generated $1,365 ha−1 more (P < 0.05). Although transgenic tobacco cultivars are currently not commercially acceptable, breeding strategies designed to reduce the amount of N. glutinosa chromatin linked to N may increase the likelihood of developing high-yielding TMV-resistant flue-cured tobacco cultivars.

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Abbreviations

TMV:

Tobacco mosaic virus

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Acknowledgments

The authors would like to thank Dr. Barbara Baker, USDA-ARS, for her cooperation in providing us with the N construct pTG34. Lori Whittock was supported by a graduate assistantship provided by Profigen. The authors are also grateful to Philip Morris USA for their support of the overall tobacco breeding and genetics research program at NCSU.

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  1. Crop Science Department, North Carolina State University, Campus Box 7620, Raleigh, NC, 27695, USA

    R. S. Lewis & E. A. Wernsman

  2. 27 Parfait Lane, Alameda, CA, 94502-6546, USA

    L. R. Linger

  3. 436 Farmington Woods Drive, Cary, NC, 27511, USA

    M. F. Wolff

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  1. R. S. Lewis
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  2. L. R. Linger
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  3. M. F. Wolff
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Correspondence to R. S. Lewis.

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Communicated by D. A. Lightfoot.

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Lewis, R.S., Linger, L.R., Wolff, M.F. et al. The negative influence of N-mediated TMV resistance on yield in tobacco: linkage drag versus pleiotropy. Theor Appl Genet 115, 169–178 (2007). https://doi.org/10.1007/s00122-007-0552-y

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