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Fine structural localization of aspartate carbamoyltransferase in rat liver

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Summary

A new procedure for demonstrating aspartate carbamoyltransferase (carbamoyl phosphate: L aspartate carbamoyltransferase, E. C.2.1,3.2) is described. The method is based on precipitating the released orthophosphate by lead ions. The resulting lead phosphate deposits serve for electron microscopic localization of aspartate carbamoyltransferase. There is a correlation of the morphological demonstration at rough endoplasmic reticulum and the biochemical determination in the microsomal fraction. Enzyme activity is found at the nucleus too.

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Authors and Affiliations

  1. Pharmakologisches Institut der Freien Universität Berlin, Germany

    S. Spors

  2. II. Anatomisches Institut der Freien Universität Berlin, Germany

    H. -J. Merker

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  1. S. Spors
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  2. H. -J. Merker
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Spors, S., Merker, H.J. Fine structural localization of aspartate carbamoyltransferase in rat liver. Histochemie 17, 78–82 (1969). https://doi.org/10.1007/BF00306333

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  • DOI: https://doi.org/10.1007/BF00306333

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