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1H, 13C and 15N NMR assignments of Mg2+ bound form of UV inducible transcript protein (UVI31+) from Chlamydomonas reinhardtii

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Abstract

Almost complete sequence specific 1H, 13C and 15N resonance assignments of Mg2+ bound form of UV inducible transcript protein (UVI31+) from Chlamydomonas reinhardtii are reported, as a prelude to its structural and functional characterization.

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References

  • Atreya H, Sahu S, Chary K, Govil G (2000) A tracked approach for automated NMR assignments in proteins (TATAPRO). J Biomol NMR 17(2):125–136

    Article  Google Scholar 

  • Atreya H, Chary K, Govil G (2002) Automated NMR assignments of proteins for high throughput structure determination: TATAPRO ii. Curr Sci Bangalore 83(11):1372–1375

    Google Scholar 

  • Barnwal RP, Chary K (2008) An efficient method for secondary structure determination in polypeptides by NMR. Curr Sci 94(10):1302–1306

    Google Scholar 

  • Barnwal RP, Rout AK, Chary KV, Atreya HS (2007) Rapid measurement of 3J (HN–Hα) and 3J (N–Hβ) coupling constants in polypeptides. J Biomol NMR 39(4):259–263

    Google Scholar 

  • Barzilay G, Mol CD, Robson CN, Walker LJ, Cunningham RP, Tainer JA, Hickson ID (1995) Identification of critical active-site residues in the multifunctional human DNA repair enzyme HAP1. Nat Struct Mol Biol 2(7):561–568

    Article  Google Scholar 

  • Bax A, Grzesiek S (1993) Methodological advances in protein NMR. Acc Chem Res 26(4):131–138

    Article  Google Scholar 

  • Bax A, Ikura M (1991) An efficient 3D NMR technique for correlating the proton and 15N backbone amide resonances with the α-carbon of the preceding residue in uniformly 15N/13C enriched proteins. J Biomol NMR 1(1):99–104

    Article  Google Scholar 

  • Beese LS, Steitz TA (1991) Structural basis for the 3′–5′ exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism. EMBO J 10(1):25

    Google Scholar 

  • Braddock DT, Louis JM, Baber JL, Levens D, Clore GM (2002) Structure and dynamics of KH domains from FBP bound to single-stranded DNA. Nature 415(6875):1051–1056

    Article  ADS  Google Scholar 

  • Chary KVR, Govil G (2008) NMR in biological systems: from molecules to human, vol 6. Springer, Berlin

  • Chary KVR, Ramakxishna H, Rama Rao KS, Murali Dhar G, Kanta Rao P (1991) Hydrodesulfurization on MoS2/MgO. Catal Lett 10(1–2):27–33

    Google Scholar 

  • Edison AS, Abildgaard F, Westler WM, Mooberry ES, Markley JL (1994) [1] Practical introduction to theory and implementation of multinuclear, multidimensional nuclear magnetic resonance experiments. Method Enzymol 239:3–79

    Article  Google Scholar 

  • Hartwig A (2001) Role of magnesium in genomic stability. Mutat Res 475(1):113–121

    Article  MathSciNet  Google Scholar 

  • Kasai T, Inoue M, Koshiba S, Yabuki T, Aoki M, Nunokawa E, Seki E, Matsuda T, Matsuda N, Tomo Y (2004) Solution structure of a BolA-like protein from Mus musculus. Protein Sci 13(2):545–548

    Article  Google Scholar 

  • Keeler J (2013) Understanding NMR spectroscopy. Wiley.com

  • Kim SH, Kim M, Lee JK, Kim MJ, Jin YH, Seong RH, Hong SH, Joe CO, Park SD (1997) Identification and expression of UVI31+, a UV-inducible gene from schizosaccharomyces pombe. Environ Mol Mutagen 30(1):72–81

    Article  Google Scholar 

  • Kim MJ, Kim HS, Lee JK, Lee CB, Park S (2002) Regulation of septation and cytokinesis during resumption of cell division requires UVI31+, a UV-inducible gene of fission yeast. Mol Cell 14(3):425–430

    MathSciNet  Google Scholar 

  • Koussevitzky S, Nott A, Mockler TC, Hong F, Sachetto-Martins G, Surpin M, Lim J, Mittler R, Chory J (2007) Signals from chloroplasts converge to regulate nuclear gene expression. Science 316(5825):715–719

    Article  ADS  Google Scholar 

  • Lee JK, Park EJ, Chung HK, Hong SH, Joe CO, Park SD (1994) Isolation of UV-inducible transcripts from Schizosaccharomyces pombe. Biochem Biophys Res Commun 202(2):1113–1119

    Article  Google Scholar 

  • Linka N, Hurka H, Lang BF, Burger G, Winkler HH, Stamme C, Urbany C, Seil I, Kusch J, Neuhaus HE (2003) Phylogenetic relationships of non-mitochondrial nucleotide transport proteins in bacteria and eukaryotes. Gene 306:27–35

    Article  Google Scholar 

  • Moharikar S, D’Souza JS, Kulkarni AB, Rao BJ (2006) Apoptotic-like cell death pathway is induced in unicellular chlorophyte Chlamydomonas reinhardtii (chlorophyceae) cells following UV irradiation: detection and functional analyses 1. J Phycol 42(2):423–433

    Article  Google Scholar 

  • Muhandiram D, Xu GY, Kay LE (1993) An enhanced-sensitivity pure absorption gradient 4D 15N, 13C-edited NOESY experiment. J Biomol NMR 3(4):463–470

    Article  Google Scholar 

  • Nakamura H, Oda Y, Iwai S, Inoue H, Ohtsuka E, Kanaya S, Kimura S, Katsuda C, Katayanagi K, Morikawa K (1991) How does RNA se H recognize a DNA. RNA hybrid? Proc Natl Acad Sci 88(24):11535–11539

    Article  ADS  Google Scholar 

  • O’Donovan A, Wood RD (1993) Identical defects in DNA repair in xeroderma pigmentosum group G and rodent ERCC group 5

  • Rout AK, Minda R, Peri D, Ramakrishnan V, Bhattacharjee S, Rao B, Chary K (2010) Sequence specific 1H, 13C and 15N backbone resonance assignments of UVI31+ from Chlamydomonas reinhardtii. Biomol NMR Assign 4(2):171–174

    Article  Google Scholar 

  • Shukla M, Minda R, Singh H, Tirumani S, Chary KV, Rao BJ (2012) UVI31+ is a DNA endonuclease that dynamically localizes to chloroplast pyrenoids in C. reinhardtii. PLoS One 7(12):e51913

    Article  ADS  Google Scholar 

  • Singh H, Raghavan V, Shukla M, Rao BJ, Chary KV (2012) 1H, 13C and 15N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii. Biomol NMR Assign:1–4

  • Singh H, Rao B, Chary KV (2013a) 1H, 13C and 15N NMR assignments of a mutant of UV inducible transcript (S55A–UVI31+) from Chlamydomonas reinhardtii. Biomol NMR Assign:1–4

  • Singh H, Shukla M, Rao BJ, Chary KV (2013b) Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins. Chem Commun 49(97):11403–11405

    Article  Google Scholar 

  • Siomi H, Choi M, Siomi MC, Nussbaum RL, Dreyfuss G (1994) Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome. Cell 77(1):33–39

    Article  Google Scholar 

  • Sirover MA, Loeb L (1977) On the fidelity of DNA replication. Effect of metal activators during synthesis with avian myeloblastosis virus DNA polymerase. J Biol Chem 252(11):3605–3610

    Google Scholar 

  • Spera S, Bax A (1991) Empirical correlation between protein backbone conformation and C. alpha and C. beta 13C nuclear magnetic resonance chemical shifts. J Am Chem Soc 113(14):5490–5492

    Article  Google Scholar 

  • Welsh KM, Lu A-L, Clark S, Modrich P (1987) Isolation and characterization of the Escherichia coli mutH gene product. J Biol Chem 262(32):15624–15629

    Google Scholar 

  • Zheng L, Li M, Shan J, Krishnamoorthi R, Shen B (2002) Distinct roles of two Mg2+ binding sites in regulation of murine flap endonuclease-1 activities. Biochemistry 41(32):10323–10331

    Article  Google Scholar 

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Acknowledgments

The facilities provided by National Facility for High-Field NMR at Tata Institute of Fundamental Research, Mumbai, supported by Department of Science and Technology (DST), New Delhi, Department of Biotechnology, New Delhi, Council of Scientific and Industrial Research, New Delhi and Tata Institute of Fundamental Research, Mumbai are acknowledged. KVRC and BJ thank DST for their JC Bose Fellowships (Governement of India).

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Authors and Affiliations

  1. Department of Chemical Sciences, Tata Institute of Fundamental Research, 1, Homi Bhabha Road, Colaba, Bombay, 400005, India

    Himanshu Singh, Deepshikha Verma & Kandala V. R. Chary

  2. Department of Biological Sciences, Tata Institute of Fundamental Research, Bombay, India

    B. J. Rao

  3. Tata Institute of Fundamental Research, Center for Interdisciplinary Sciences, 21, Brindavan Colony, Narsingi, Hyderabad, 500075, India

    Kandala V. R. Chary

Authors
  1. Himanshu Singh
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  2. Deepshikha Verma
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  3. B. J. Rao
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  4. Kandala V. R. Chary
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Correspondence to Kandala V. R. Chary.

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Singh, H., Verma, D., Rao, B.J. et al. 1H, 13C and 15N NMR assignments of Mg2+ bound form of UV inducible transcript protein (UVI31+) from Chlamydomonas reinhardtii . Biomol NMR Assign 9, 93–97 (2015). https://doi.org/10.1007/s12104-014-9551-5

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