Abstract
Ribosome biogenesis is an energetically expensive and complex cellular process that involves the coordinated folding of the ribosomal RNA and dozens of ribosomal proteins. It proceeds along multiple parallel pathways and is guided by trans-acting factors called ribosome assembly factors. Although this process has been studied for decades, there are still many open questions regarding the role of the ribosome assembly factors in directing the folding of ribosome biogenesis intermediates. RimP is one of the early acting factors and guides the assembly of the small 30S ribosomal subunit by facilitating the binding of ribosomal proteins uS5 and uS12. Here we report the virtually complete 1H, 15N, and 13C chemical shift assignment of RimP from Escherichia coli. The NMR chemical shift data, deposited in the BMRB data bank under Accession No. 28014, indicates a widely folded protein composed of three alpha helices and eight beta strands.
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Abbreviations
- IPTG:
-
Isopropyl-thio-β-d-galactoside
- E. coli:
-
Escherichia coli
- RimP:
-
Ribosome maturation factor P
- PIC:
-
Protease inhibitor cocktail
- TCEP:
-
Tris(2-carboxyethyl)-phosphine
- RCI:
-
Random coil index
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Acknowledgements
This work was supported by grants from the European Union’s Seventh Framework Program (Marie Curie Actions; COFUND; to S.R.C. and A.S.), Marie Curie Action Career Integration Grant (PCIG14-GA-2013-632072 to P.F.), and Ministerio de Economía Y Competitividad Grant (MINECO, CTQ2017-82222-R to P.F., S.R.C.). We thank MINECO for the Severo Ochoa Excellence Accreditation (SEV-2016-0644) and the proteomics platform of CIC bioGUNE for sample analysis by MALDI TOF mass spectroscopy.
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Schedlbauer, A., Ochoa-Lizarralde, B., Iturrioz, I. et al. Backbone and sidechain NMR assignments for the ribosome maturation factor RimP from Escherichia coli. Biomol NMR Assign 14, 189–193 (2020). https://doi.org/10.1007/s12104-020-09943-w
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DOI: https://doi.org/10.1007/s12104-020-09943-w