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An enhanced-sensitivity pure absorption gradient 4D 15N, 13C-edited NOESY experiment

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Summary

An enhanced-sensitivity gradient 4D 15N, 13C-edited NOESY experiment is presented. Gradients are employed to suppress artifacts, eliminate the intense H2O signal and select for the coherence transfer pathway involving 15N magnetization. The latter use of the gradients results in a decrease in the number of phase cycle steps by a factor of two relative to the previously published 4D sequence (Kay et al., 1990) allowing for the recording of spectra with increased resolution per unit measuring time. Theoretical sensitivity enhancements of as much as a factor of \(\sqrt 2 \) can be expected over the previously published sequence, neglecting the effects of relaxation and pulse imperfections.

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Authors and Affiliations

  1. Protein Engineering Network Centres of Excellence, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    D. R. Muhandiram, Guang Yi Xu & Lewis E. Kay

  2. Department of Medical Genetics, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    D. R. Muhandiram, Guang Yi Xu & Lewis E. Kay

  3. Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    D. R. Muhandiram, Guang Yi Xu & Lewis E. Kay

  4. Department of Chemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    D. R. Muhandiram, Guang Yi Xu & Lewis E. Kay

Authors
  1. D. R. Muhandiram
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  2. Guang Yi Xu
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  3. Lewis E. Kay
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Muhandiram, D.R., Xu, G.Y. & Kay, L.E. An enhanced-sensitivity pure absorption gradient 4D 15N, 13C-edited NOESY experiment. J Biomol NMR 3, 463–470 (1993). https://doi.org/10.1007/BF00176011

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  • DOI: https://doi.org/10.1007/BF00176011

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