Abstract
Sacha inchi (Plukenetia volubilis L., Euphorbiaceae) seed oil is rich in α-linolenic acid, a kind of n-3 fatty acids with many health benefits. To discover the mechanism underlying α-linolenic acid accumulation in sacha inchi seeds, preliminary research on sacha inchi seed development was carried out from one week after fertilization until maturity, focusing on phenology, oil content, and lipid profiles. The results suggested that the development of sacha inchi seeds from pollination to mature seed could be divided into three periods. In addition, investigations on the effect of temperature on sacha inchi seeds showed that total oil content decreased in the cool season, while unsaturated fatty acid and linolenic acid concentrations increased. In parallel, expression profiles of 17 unsaturated fatty acid related genes were characterized during seed development and the relationships between gene expression and lipid/unsaturated fatty acid accumulation were discussed.
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Abbreviations
- ALA:
-
Alpha-linolenic acid (18:3n-3)
- DAP:
-
Days after pollination
- DGAT:
-
Acyl-CoA: diacylglycerol acyltransferase
- DW:
-
Dry weight
- FA:
-
Fatty acid desaturase
- FATA:
-
Acyl-acyl-carrier protein thioesterase A
- FFA:
-
Free fatty acids
- FW:
-
Fresh weight
- G3PDH:
-
Glycerol-3-phosphate dehydrogenase
- LNA:
-
Linoleic acid (18:2n-6)
- MBOAT:
-
Membrane-bound O-acyltransferase
- NL:
-
Neutral lipids
- OLA:
-
Oleic acid (18:1n-9)
- PAM:
-
Palmitic acid (16:0)
- PAP:
-
Phosphatidate phosphatase
- PL:
-
Phospholipids
- PLA2 :
-
Phospholipase A2
- PLC:
-
Phospholipase C
- PUFA:
-
Polyunsaturated fatty acid(s)
- SAD:
-
Stearoyl-acyl-carrier protein desaturase
- SFA:
-
Saturated fatty acid(s)
- STA:
-
Stearic acid (18:0)
- TAG:
-
Triacylglycerol(s)
- UI:
-
Unsaturation index
- USFA:
-
Unsaturated fatty acid(s)
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Acknowledgments
We thank the Seed Bank of Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, and Xishuangbanna Station for Tropical Rain Forest Ecosystem Studies (XSTRE) for assistance in sample collection and atmospheric temperature observation. We also thank Dr. Hongtao Li from the SW China Germplasm Bank of Wild Species, Kunming Institute of Botany for his assistance in conducting quantitative real-time PCR. This work was financially supported by the Hundreds of Talents Program from Chinese Academy of Sciences (To AL).
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We declare that we have no competing interests.
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Wang, X., Liu, A. Expression of Genes Controlling Unsaturated Fatty Acids Biosynthesis and Oil Deposition in Developing Seeds of Sacha Inchi (Plukenetia volubilis L.). Lipids 49, 1019–1031 (2014). https://doi.org/10.1007/s11745-014-3938-z
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DOI: https://doi.org/10.1007/s11745-014-3938-z