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Characterization of sacha inchi (Plukenetia volubilis L.) oil by FTIR spectroscopy and 1H NMR. Comparison with linseed oil

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Journal of the American Oil Chemists' Society

Abstract

Three oil samples obtained from sacha inchi (Plukenetia volubilis L.) seeds were studied by means of FTIR and 1H NMR. Frequency data of the most significant bands of the IR spectrum of this oil are given. These data show that sacha inchi oil has a high degree of unsaturation. The same fact is deduced from the ratio between the absorbance of the bands due to the stretching vibrations of the cis olefinic CH double bonds at 3010.5 cm−1 and to the methylene symmetrical stretching vibrations at 2855.1 cm−1. The proportions of monounsaturated, polyunsaturated, and saturated acyl groups were predicted from the frequency of some IR bands, and these were in satisfactory agreement with the values obtained through FAME generation and their quantification by GC. Likewise, simple observation of the 1H NMR spectra provided a great deal of information about sacha inchi oil, with regard not only to the relative proportions of the different acyl groups but also to their nature. Thus, the presence of γ-linolenic acyl groups was discounted. Furthermore, the area of some 1H NMR signals was used to determine the proportion of saturated and mono-, di-, and triunsaturated acyl groups, which also were in satisfactory agreement with the values obtained by classical methods. IR and 1H NMR determinations take very little time in comparison with classical methods and do not require chemical manipulation or transformation of the sample. A comparison was also made between the compositions of sacha inchi and linseed oil. Both oils are important sources of the healthful n−3 linolenic acyl groups, and sacha inchi also contains high proportions of the n−6 linoleic acyl groups.

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References

  1. Hamaker, B.R., C. Valles, R. Gilman, R.M. Hardmeier, D. Clark, H.H. García, A.E. Gonzales, I. Kohlstad, and M. Castro, Amino Acid and Fatty Acid Profiles of the Inca Peanut (Plukenetia volubilis L.), Cereal Chem. 69:461–463 (1992).

    CAS  Google Scholar 

  2. Simopoulos, A.P., Omega-3 Fatty Acids in Health and Disease and in Growth and Development, Am. J. Clin. Nutr. 34:411–414 (1991).

    Google Scholar 

  3. Fernandes, G., and J.T. Venkatraman, Role of Omega-3 Fatty Acids in Health and Disease, Nutr. Res. 13 (Suppl. 1):S19-S45 (1993).

    Article  CAS  Google Scholar 

  4. Guillén, M.D., and N. Cabo, Infrared Spectroscopy in the Study of Edible Oils and Fats, J. Sci. Food Agric. 75:1–11 (1997).

    Article  Google Scholar 

  5. Guillén, M.D., and A. Ruiz, High Resolution 1H Nuclear Magnetic Resonance in the Study of Edible Oils and Fats, Trends Food Sci. Technol. 12:328–338 (2001).

    Article  Google Scholar 

  6. Van de Voort, F.R., A.A. Ismail, J. Sedman, G. Emo, and A.A. Ismail, A Rapid FTIR Quality Control Method for Fat and Moisture Determination in Butter, Food Res. Int. 25:193–198 (1992).

    Article  Google Scholar 

  7. Van de Voort, F.R., J. Sedman, G. Emo, and A.A. Ismail, Rapid and Direct Iodine Value and Saponification Number Determination of Fats and Oils by Attenuated Total Reflectance/Fourier Transform Infrared Spectroscopy, J. Am. Oil Chem. Soc. 69: 1118–1123 (1992).

    Google Scholar 

  8. Van de Voort, F.R., A.A. Ismail, J. Sedman, J. Dubois, and T. Nicodemo, The Determination of Peroxide Value by Fourier Transform Infrared Spectroscopy, Ibid. 71:921–926 (1994).

    Google Scholar 

  9. Van de Voort, F.R., A.A. Ismail, and J. Sedman, A Rapid, Automated Method for the Determination of cis and trans Content of Fats and Oils by Fourier Transform Infrared Spectroscopy, Ibid. 72:873–880 (1995).

    Google Scholar 

  10. Safar, M., D. Bertrand, P. Robert, M.F. Devaux, and C. Genot, Characterization of Edible Oils, Butters, and Margarines by Fourier Transform Infrared Spectroscopy with Attenuated Total Reflectance, Ibid. 71:371–377 (1994).

    CAS  Google Scholar 

  11. Guillén, M.D., and N. Cabo, Characterization of Edible Oils and Lard by Fourier Transform Infrared Spectroscopy. Relationships Between Composition and Frequency of Concrete Bands of the Fingerprint Region, Ibid. 74:1281–1286 (1997).

    Google Scholar 

  12. Guillén, M.D., and N. Cabo, Relationships Between the Composition of Edible Oils and Lard and the Ratio of the Absorbance of Specific Bands of Their Fourier Transform Infrared Spectra. Role of Some Bands of the Fingerprint Region, J. Agric. Food Chem. 46:1788–1793 (1998).

    Article  Google Scholar 

  13. Guillén, M.D., and N. Cabo, Usefulness of the Frequencies of Some Fourier Transform Infrared Spectroscopic Bands for Evaluating the Composition of Edible Oil Mixtures, Fett/Lipid 101:71–76 (1999).

    Article  Google Scholar 

  14. Guillén, M.D., and N. Cabo, Characterization of Edible Oils and Fats and Determination of Their Oxidative Stability by Means of the Frequency Value of Specific Bands of Their Infrared Spectrum, Alimentaria 7–8:51–58 (2000).

    Google Scholar 

  15. Sacchi, R., M. Patumi, G. Fontanazza, P. Barone, P. Fiodiponti, L. Mannina, E. Rossi, and A.L. Segre, A High-Field 1H Nuclear Magnetic Resonance Study of the Minor Components in Virgin Olive Oils, J. Am. Oil Chem. Soc. 73:747–758 (1996).

    Article  CAS  Google Scholar 

  16. Segre, A.L., and L. Mannina, 1H NMR Study of Edible Oils, Rec. Res. Devel. Oil Chem. 1:297–308 (1997).

    CAS  Google Scholar 

  17. Sacchi, R., L. Mannina, P. Fiordiponti, P. Barone, L. Paolillo, M. Patumi, and A.L. Segre, Characterization of Italian Extra Virgin Olive Oils Using 1H NMR Spectroscopy, J. Agric. Food Chem. 46:3947–3951 (1998).

    Article  CAS  Google Scholar 

  18. Mannina, L., M. Patumi, P. Fiordiponti, M.C. Emanuele, and A.L. Segre, Olive and Hazelnut Oils: A Study by High-Field 1H NMR and Gas Chromatography, Ital. J. Food Sci. 2:139–149 (1999).

    Google Scholar 

  19. Sacco, A., M.A. Brescia, V. Liuzzi, F. Reniero, C. Guillou, S. Ghelli, and P. Van der Meer, Characterization of Italian Olive Oils Based on Analytical and Nuclear Magnetic Resonance Determinations, J. Am. Oil Chem. Soc. 77:619–625 (2000).

    CAS  Google Scholar 

  20. Johnson, L.F., and J.N. Schoolery, Determination of Unsaturation and Average Molecular Weight of Natural Fats by Nuclear Magnetic Resonance, Anal. Chem. 34:1136–1139 (1962).

    Article  CAS  Google Scholar 

  21. Guillén, M.D., M.L. Ibargoitia, N. Cabo, and A. Ruiz, 1H NMR Monitoring of Safflower Oil Degradation Under Several Oxidation Conditions, in 5th ICAMRFS—The 5th International Conference Oil Application of Magnetic Resonance, University of Aveiro, Aveiro, Portugal, 2000.

  22. Guillén, M.D., and A. Ruiz, Edible Oils: Discrimination by 1H Nuclear Magnetic Resonance, J. Sci. Food Agric. 83:338–346 (2003).

    Article  Google Scholar 

  23. European Economic Community, Regulation EEC/2568/91 on the Characteristics of Olive and Olive Pomace Oils and on Their Analytical Methods. Annexe XB Preparation of the Methyl Esters of Fatty Acids, Off. J. Eur. Commun. L 248:111–114 (1991).

    Google Scholar 

  24. Afran, A., and E.J. Newbery, Analysis of the Degree of Unsaturation in Edible Oils by Fourier Transform Infrared Attenuated Total Reflectance Spectroscopy, Spectroscopy 6:31–34 (1991).

    CAS  Google Scholar 

  25. Arnold, R.G., and T.E. Hartung, Infrared Spectroscopy Determination of Degree of Unsaturation of Fats and Oils, J. Food Sci. 36:166–168 (1971).

    Article  CAS  Google Scholar 

  26. Mikusch, J.D., The Specifications for Linseed Oils, Farbe + Lack 57:91–94 (1951).

    CAS  Google Scholar 

  27. Aursand, M., J.R. Rainuzzo, and H. Grasladen, Quantitative High-Resolution 13C and 1H Nuclear Magnetic Resonance of ω-3 Fatty Acids from White Muscle of Atlantic Salmon (Salmo salar), J. Am. Oil Chem. Soc. 70:971–981 (1993).

    CAS  Google Scholar 

  28. Sacchi, R., I. Medina, S.P. Aubourg, F. Addeo, and L. Paolillo, Proton Nuclear Magnetic Resonance Rapid and Structure-Specific Determination of ω-3 Polyunsaturated Fatty Acids in Fish Lipids, Ibid. 70:225–228 (1993).

    CAS  Google Scholar 

  29. Igarashi, T., M. Aursand, Y. Hirata, I.S. Gribbestad, S. Wada, and M. Nonaka, Nondestructive Quantitative Determination of Docosahexaenoic Acid and n−3 Fatty Acids in Fish Oils by High-Resolution 1H Nuclear Magnetic Resonance Spectroscopy, Ibid. 77:737–748 (2000).

    CAS  Google Scholar 

  30. Pouchert, C.J., and J. Behnke, The Aldrich Library of 13 C and 1 H FT-NMR Spectra, 1st edn., Aldrich Chemical Company, Milwaukee, 1993, Vol. 1.

    Google Scholar 

  31. Ketshajwang, K.K., J. Holmback, and S.O. Yeboah, Quality and Compositional Studies of Some Edible Leguminosae Seed Oils in Botswana, J. Am. Oil Chem. Soc. 75:741–743 (1998).

    CAS  Google Scholar 

  32. Miyake, Y., K. Yokomizo, and N. Matsuzaki, Determination of Unsaturated Fatty Acid Composition by High-Resolution Nuclear Magnetic Resonance Spectroscopy, Ibid. 75:1091–1094 (1998).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Universidad Agraria de La Molina, Lima, Perú

    Rosana Chirinos & Gloria Pascual

  2. Tecnología de Alimentos, Facultad de Farmacia, Universidad del País Vasco, Paseo de la Universidad 7, 01006, Vitoria, Spain

    María D. Guillén, Ainhoa Ruiz & Nerea Cabo

Authors
  1. María D. Guillén
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  2. Ainhoa Ruiz
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  3. Nerea Cabo
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  4. Rosana Chirinos
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  5. Gloria Pascual
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Correspondence to María D. Guillén.

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Guillén, M.D., Ruiz, A., Cabo, N. et al. Characterization of sacha inchi (Plukenetia volubilis L.) oil by FTIR spectroscopy and 1H NMR. Comparison with linseed oil. J Amer Oil Chem Soc 80, 755–762 (2003). https://doi.org/10.1007/s11746-003-0768-z

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  • DOI: https://doi.org/10.1007/s11746-003-0768-z

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