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Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum)

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Abstract

NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. In the current study, expression profiles of wheat NAC-type transcription factor genes, TaNAC69-1 and TtNAMB-2, were examined under drought, salt, cold, and heat stress conditions in wheat. Based on reverse transcription quantitative PCR results, TaNAC69-1 was strongly expressed under drought, salinity, and high-temperature stress conditions. Compared to control samples, a quick response at the transcription level of TaNAC69-1 was observed after 3 h of salt treatment with a ninefold upregulation. Highest level of expression was observed at 24 and 48 h posttreatment under heat and salinity treatments, respectively. Meanwhile, expression of TtNAMB-2 was significantly induced by salt and low-temperature stresses. Salt treatment induced expression of TtNAMB-2 and caused a 13-fold increase in transcript copy numbers at 48 h posttreatment. Examination of expression changes under abiotic stresses may provide important information for understanding roles of TaNAC69-1 and TtNAMB-2 genes which might be involved in response to environmental stresses.

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Acknowledgments

We would like to thank METU Central Laboratory, Molecular Biology and Biotechnology R&D Center for analysis of RNA samples. This study was supported by the Research Fund of METU grant no. BAP-08-11-DPT2002K120510 and TÜBİTAK grant no. 108O786.

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Authors and Affiliations

  1. Department of Biological Sciences, Middle East Technical University, Üniversiteler Mah. Dumlupınar Blv. No. 1, 06800, Ankara, Turkey

    Mehmet Cengiz Baloglu, Mehmet Tufan Oz, Huseyin Avni Oktem & Meral Yucel

  2. Department of Biology, Kastamonu University, 37100, Kuzeykent, Kastamonu, Turkey

    Mehmet Cengiz Baloglu

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  1. Mehmet Cengiz Baloglu
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  2. Mehmet Tufan Oz
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Correspondence to Meral Yucel.

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Baloglu, M.C., Oz, M.T., Oktem, H.A. et al. Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum). Plant Mol Biol Rep 30, 1246–1252 (2012). https://doi.org/10.1007/s11105-012-0445-3

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