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Cloning and Characterization of a Novel NAC Family Gene CarNAC1 from Chickpea (Cicer arietinum L.)

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An Erratum to this article was published on 11 February 2016

Abstract

The plant-specific NAC (for NAM, ATAF1,2 and CUC2) proteins have been found to play important roles in plant development and stress responses. In this study, a NAC gene CarNAC1 (for Cicer arietinum L. NAC gene 1) was isolated from a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol. CarNAC1 encoded a putative protein with 239 amino acids and contained 3 exons and 2 introns within genomic DNA sequence. CarNAC1 had a conserved NAC domain in the N-terminus and the CarNAC1:GFP (green fluorescent protein) fusion protein was localized in the nucleus of onion epidermal cells. Additionally, CarNAC1 exhibited the trans-activation activity which was mapped to the C-terminus. The CarNAC1 transcript was detected in many chickpea organs including seedling leaves, stems, roots, flowers, and young pods, but less accumulated in young seeds. CarNAC1 was induced by leaf age and showed changes in expression during seed development and germination. Furthermore, the expression of CarNAC1 was strongly induced by drought, salt, cold, wounding, H2O2, ethephon, salicylic acid, indole-3-acetic acid, and gibberellin. Our results suggest that CarNAC1 encodes a novel NAC-domain protein and may be a transcriptional activator involved in plant development and various stress responses.

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Abbreviations

ABA:

Abscisic acid

6-BA:

N-6-benzyl-adenine

CUC:

Up-shaped cotyledon

DAF:

Days after flowering

EST:

Expressed sequence tag

ET:

Ethephon

GA3:

Gibberellin

GFP:

Green fluorescent protein

IAA:

Indole-3-acetic acid

MeJA:

Methyl jasmonate

NAC:

NAM, ATAF1,2 and CUC2

NST:

NAC secondary wall thickening promoting factor

ORF:

Open reading frame

PEG:

Polyethylene glycol

RACE:

Rapid amplification of cDNA end

RT-PCR:

Reverse transcription polymerase chain reaction

SA:

Salicylic acid

SD:

Synthetic defined medium

SENU5:

Senescence up-regulated 5

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Acknowledgments

We gratefully acknowledge the financial support from the 111 Project supported by the Ministry of Education of China, from projects supported by the National Science and Technology Ministry of China (2007BAC15B06, 2006BAD09A04, 2006BAD09A08), from a project supported by the National Natural Science Foundation of China (30860152), from a project supported by the Xinjiang Science and Technology Department of China (200991254), and from a project supported by the Key Teacher Foundation of Guangxi Normal University, China, for this research. We also thank Prof. Lizhong Xiong from Huazhong Agricultural University, China, for kindly supplying the trans-activation system.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, China

    Hui Peng, Xingwang Yu, Huiying Cheng & Hao Ma

  2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, 541004, China

    Hui Peng

  3. Key Laboratory of Agricultural Biotechnology, Xinjiang Agricultural University, Urumqi, 830052, China

    Qinghua Shi, Hua Zhang & Jiangui Li

Authors
  1. Hui Peng
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  2. Xingwang Yu
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  4. Qinghua Shi
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  7. Hao Ma
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Corresponding author

Correspondence to Hao Ma.

Additional information

H. Peng and X. Yu contributed equally to this study.

An erratum to this article is available at http://dx.doi.org/10.1007/s12033-016-9916-3.

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Peng, H., Yu, X., Cheng, H. et al. Cloning and Characterization of a Novel NAC Family Gene CarNAC1 from Chickpea (Cicer arietinum L.). Mol Biotechnol 44, 30–40 (2010). https://doi.org/10.1007/s12033-009-9202-8

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