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Identification and molecular mapping of a dwarfing gene in barley (Hordeum vulgare L.) and its correlation with other agronomic traits

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Abstract

Ninety-two doubled haploid (DH) lines, generated from a cross between Franklin and TX9425 (a Chinese Landrace), were grown in three environments to identify quantitative trait loci (QTLs) controlling agronomic traits including heading date, plant height and spike characteristics. The DH lines showed a wide range of variations for all the agronomic traits tested. Most of the traits were controlled by one or two major QTLs which explained 9.5–80.9% of the phenotypic variation. Two dwarfing genes were identified from the cross. One of the dwarfing genes was from Franklin, which is the same as the previously reported denso gene. The other dwarfing gene was from the Chinese landrace variety. Both dwarfing genes were temperature and/or day length sensitive. The dwarfing gene from Franklin was more effective in early sowing trials (shorter day length and lower temperature) while the gene from TX9425 was more effective in later sown trials. The dwarfing gene from TX9425 was located at a similar position to the uzu gene. However, it differed from this gene being temperature sensitive with very close links to short spikes, awns and high grain density which is more like a brh gene. To effectively use this gene in a breeding program, it is necessary to break the linkage between the dwarfing gene and the unfavourable spike traits.

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References

  • Abeledo LG, Calderini DF, Slafer GA (2002) Physiological changes associated with genetic improvement of grain yield barley. In: Slafer GA, Molina-Cano JL, Savin R, Araus JL, Romagosa I (eds) Barley science: recent advances from molecular biology to agronomy of yield and quality. The Haworth Press Inc., Binghamton, New York, pp 361–386

    Google Scholar 

  • Backes G, Graner A, Foroughi-Wehr B, Fischbeck G, Wenzel G, Jahoor A (1995) Localization of quantitative trait loci (QTL) for agronomic important characters by the use of a RFLP map in barley (Hordeum vulgare L.). Theor Appl Genet 90:294–302

    Article  CAS  Google Scholar 

  • Baghizadeh A, Taleei AR, Naghavi MR (2007) QTL analysis for some agronomic traits in barley (Hordeum vulgare L). Int J Agr Biol 9:372–374

    Google Scholar 

  • Bezant J, Laurie D, Pratchett N, Chojecki J, Kearsey M (1996) Marker regression mapping of QTL controlling flowering time and plant height in a spring barley (Hordeum vulgare L.) cross. Heredity 77:64–73

    Article  CAS  Google Scholar 

  • Chen Z, Zhou M, Newman IA, Mendham NJ, Zhang G, Shabala S (2007) Potassium and sodium relations in salinised barley tissues as a basis of differential salt tolerance. Funct Plant Biol 34:150–162

    Article  CAS  Google Scholar 

  • Dahleen LS, LJvd Wal, Franckowiak JD (2005) Characterization and Molecular Mapping of genes determining semidwarfism in barley. J Hered 96:654–662

    Article  CAS  PubMed  Google Scholar 

  • Davies PA, Morton S (1998) A comparison of barley isolated microspore and anther culture and the influence of the cell culture density. Plant Cell Rep 17:206–210

    Article  CAS  Google Scholar 

  • Hayes PM, Liu BH, Knapp SJ, Chen F, Jones B, Blake T, Franckowiak J, Rasmusson D, Sorrells M, Ullrich SE, Wesenberg D, Kleinhofs A (1993) Quantitative trait locus effects and environmental interaction in a sample of North American barley germplasm. Theor Appl Genet 87:392–401

    Article  Google Scholar 

  • Hellewell KB, Rasmusson DC, Gallo-Meagher M (2000) Enhancing yield of semidwarf barley. Crop Sci 40:352–358

    Article  Google Scholar 

  • Hori K, Kobayashi T, Shimizu A, Sato K, Takeda K, Kawasaki S (2003) Efficient construction of high-density linkage map and its application to QTL analysis in barley. Theor Appl Genet 107:806–813

    Article  CAS  PubMed  Google Scholar 

  • Jaccoud D, Peng KM, Feinstein D, Kilian A (2001) Diversity arrays: a solid state technology for sequence information independent genotyping. Nucleic Acids Res 29:e25

    Article  CAS  PubMed  Google Scholar 

  • Kjær B, Jenson J, Giese H (1995) Quantitative trait loci for heading date and straw characters in barley. Genome 38:1098–1104

    PubMed  Google Scholar 

  • Laurie DA, Pratchett N, Romero C, Simpson E, Snape JW (1993) Assignment of the denso dwarfing gene to the long arm of chromosome 3 (3H) of barley by use of RFLP markers. Plant Breed 111:198–203

    Article  Google Scholar 

  • Li JZ, Huang XQ, Heinrichs F, Ganal MW, Roder MS (2005) Analysis of QTLs for yield, yield components, and malting quality in a BC3-DH population of spring barley. Theor Appl Genet 110:356–363

    Article  CAS  PubMed  Google Scholar 

  • Li H, Vaillancourt R, Mendham N, Zhou M (2008) Comparative mapping of quantitative trait loci associated with waterlogging tolerance in barley (Hordeum vulgare L.). BMC Genomics 9:401

    Article  PubMed  Google Scholar 

  • Marquez-Cedillo LA, Hayes PM, Kleinhofs A, Legge WG, Rossnagel BG, Sato K, Ullrich SE, Wesenberg DM (2001) QTL analysis of agronomic traits in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups. Theor Appl Genet 103:625–637

    Article  CAS  Google Scholar 

  • Pang J, Zhou M, Mendham N, Shabala S (2004) Growth and physiological responses of six barley genotypes to waterlogging and subsequent recovery. Aust J Agric Res 55:895–906

    Article  Google Scholar 

  • Pang JY, Mendham N, Zhou MX, Newman I, Shabala S (2006) Microelectrode ion and O2 flux measurements reveal differential sensitivity of barley root tissues to hypoxia. Plant Cell Environ 29:1107–1121

    Article  CAS  PubMed  Google Scholar 

  • Pang J, Cuin T, Shabala L, Zhou M, Mendham N, Shabala S (2007a) Effect of secondary metabolites associated with anaerobic soil conditions on ion fluxes and electrophysiology in barley roots. Plant Physiol 145:266–276

    Article  CAS  PubMed  Google Scholar 

  • Pang J, Ross J, Zhou M, Mendham N, Shabala S (2007b) Amelioration of detrimental effects of waterlogging by foliar nutrient sprays in barley. Funct Plant Biol 34:221–227

    Article  CAS  Google Scholar 

  • Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726

    Article  CAS  PubMed  Google Scholar 

  • Pillen K, Zacharias A, Leon J (2003) Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor Appl Genet 107:340–352

    Article  CAS  PubMed  Google Scholar 

  • Sameri M, Komatsuda K (2004) Identification of quantitative trait loci (QTLs) controlling heading time in the population generated from a cross oriental and occidental barley cultivars (Hordeum vulgare L). Breed Sci 54:327–334

    Article  CAS  Google Scholar 

  • Sameri M, Takeda K, Komatsuda T (2006) Quantitative trait loci controlling agronomic traits in recombinant inbred lines from a cross between oriental- and occidental-type barley cultivars. Breed Sci 56:243–252

    Article  Google Scholar 

  • Takahashi R, Yamamoto J (1951) Studies on the classification and geographycal distribution of the Japanese barley varieties. III. On the linkage relation and the region of “uzu”or semi-brachytic character in barley. Ber Ohara Inst Landow Forsch 9:399–410

    Google Scholar 

  • Takhashi R, Yasuda S (1970) Genetics of earliness and growth habit in barley. In: Nilan RA (ed) Barley Genetics II, Proceedings of the Second International Barley Genetics Symposium. Washington State University Press, Pullman, WA, pp 388–408

  • Tang QY, Feng MG (2007) DPS Data processing system: experimental design statistical analysis and data mining. Science Press, Beijing

    Google Scholar 

  • Tinker NA, Mather DE, Rossnagel BG, Kasha KJ, Kleinhofs A, Hayes PM et al (1996) Regions of the genome that affect agronomic performance in two-row barley. Crop Sci 36:1053–1062

    Article  Google Scholar 

  • Van Ooijen JW, Kyazma BV (2004) MapQTL® 5 Software for the mapping of quantitative trait loci in experimental populations. Wageningen, The Netherland

    Google Scholar 

  • Varshney RK, Marcel TC, Ramsay L, Russell J, Roder MS, Stein N, Waugh R, Langridge P, Niks RE, Graner A (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theor Appl Genet 114:1091–1103

    Article  CAS  PubMed  Google Scholar 

  • Von Korff M, Wang H, Léon J, Pillen K (2006) AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley. Theor Appl Genet 112:1221–1231

    Article  Google Scholar 

  • Wenzl P, Carling J, Kudrna D, Jaccoud D, Huttner E, Kleinhofs A, Kilian A (2004) Diversity arrays technology (DArT) for whole-genome profiling of barley. Proc Nat Acad Sci 101:9915–9920

    Article  CAS  PubMed  Google Scholar 

  • Wenzl P, Li H, Carling J, Zhou M, Raman H, Paul E, Hearnden P, Maier C, Xia L, Caig V, Ovesna J, Cakir M, Poulsen D, Wang J, Raman R, Smith KP, Muehlbauer GJ, Chalmers KJ, Kleinhofs A, Huttner E, Kilian A (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics 7:206–227

    Article  PubMed  Google Scholar 

  • Zhang J, Sun LJ (1993) Inheritance of spike length and density of barley in three crosses. Hereditas (Beijing) 15:23–26

    CAS  Google Scholar 

  • Zhou MX, Li HB, Mendham NJ (2007) Combining ability of waterlogging tolerance in barley (Hordeum vulgare L.). Crop Sci 47:278–284

    Article  Google Scholar 

  • Zhou MX, Li HB, Chen ZH, Mendham NJ (2008) Combining ability of barley flour pasting properties. J Cereal Sci 48:789–793

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by Grains Research and Development Corporation of Australia (UT8) grant to M.Z. and National Natural Science Foundation of China (30700485) grant to J.W.

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Authors and Affiliations

  1. Tasmanian Institute of Agricultural Research, University of Tasmania, P.O. Box 46, Kings Meadows, TAS, 7249, Australia

    Junmei Wang, David L. McNeil & Meixue Zhou

  2. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Junmei Wang & Jianming Yang

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  1. Junmei Wang
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  2. Jianming Yang
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  3. David L. McNeil
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  4. Meixue Zhou
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Correspondence to Meixue Zhou.

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Wang, J., Yang, J., McNeil, D.L. et al. Identification and molecular mapping of a dwarfing gene in barley (Hordeum vulgare L.) and its correlation with other agronomic traits. Euphytica 175, 331–342 (2010). https://doi.org/10.1007/s10681-010-0175-2

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