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Proteomic and morphometric study of the in vitro interaction between Oncidium sphacelatum Lindl. (Orchidaceae) and Thanatephorus sp. RG26 (Ceratobasidiaceae)

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Abstract

Orchidaceae establish symbiotic relationships with fungi in the Rhizoctonia group, resulting in interactions beneficial to both organisms or in cell destruction in one of them (pathogenicity). Previous studies have focused mostly on terrestrial species with a few, preliminary studies, on epiphytes. To further our understanding of the molecular mechanisms involved in these symbioses, we evaluated the interaction between Oncidium sphacelatum Lindl. and the mycorrhizal fungus Thanatephorus sp. strain RG26 (isolated from a different orchid species) in vitro using morphometric and proteomic analyses. Evidence from the morphometric and microscopic analysis showed that the fungus promoted linear growth and differentiation of orchid protocorms during 98 days interaction. On day 63, protocorm development was evident, so we analyzed the physiological response of both organisms at that moment. Proteome results suggest that orchid development stimulated by the fungus apparently involves cell cycle proteins, purine recycling, ribosome biogenesis, energy metabolism, and secretion that were up-regulated in the orchid; whereas in the fungus, a high expression of proteins implicated in stress response, protein-protein interaction, and saccharides and protein biosynthesis were found in the symbiotic interaction. This is the first work reporting proteins differentially expressed in the epiphytic orchid-fungus interaction and will contribute to the search for molecular markers that will facilitate the study of this symbiosis in both wild orchids and those in danger of extinction.

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Acknowledgments

We thank Guadalupe Nieto-López for the scanning electron microscopy analysis, Luis Barbosa for analysis with the MATLAB R2012a program, Alejandro Zamora for help in protein extraction and the phylogenetic reconstruction of strain RG26, and the Consejo Nacional de Ciencia y Tecnología scholarship No. 355415. We thank Michael F. Dunn for his valuable contribution to the improvement of the manuscript.

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  1. El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km 2.5, C.P. 30700, Tapachula, Chiapas, Mexico

    Mariana Yadira López-Chávez, Karina Guillén-Navarro, Vincenzo Bertolini & Anne Damon

  2. El Colegio de la Frontera Sur, Carretera Panamericana y Periférico Sur s/n, Barrio de María Auxiliadora, C.P. 29290, San Cristóbal de Las Casas, Chiapas, Mexico

    Irene Sánchez-Moreno

  3. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad s/n, Col. Chamilpa, C.P. 62210, Cuernavaca, Morelos, Mexico

    Sergio Encarnación & Magdalena Hernández-Ortiz

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  1. Mariana Yadira López-Chávez
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López-Chávez, M.Y., Guillén-Navarro, K., Bertolini, V. et al. Proteomic and morphometric study of the in vitro interaction between Oncidium sphacelatum Lindl. (Orchidaceae) and Thanatephorus sp. RG26 (Ceratobasidiaceae). Mycorrhiza 26, 353–365 (2016). https://doi.org/10.1007/s00572-015-0676-x

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