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Ceratobasidiaceae mycorrhizal fungi isolated from nonphotosynthetic orchid Chamaegastrodia sikokiana

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Abstract

Mycorrhizal fungi were isolated from the nonphotosynthetic orchid Chamaegastrodia sikokiana and identified as members of Ceratobasidiaceae by phylogenetic analysis of the internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid. The ITS sequences were similar among geographically separated samples obtained from Mt. Kiyosumi in Chiba Prefecture and Mt. Yokokura in Kochi Prefecture. One of the isolated fungi, KI1-2, formed ectomycorrhiza on seedlings of Abies firma in pot culture, suggesting that tripartite symbiosis exists among C. sikokiana, mycorrhizal fungi, and A. firma in nature, and carbon compounds are supplied from A. firma to C. sikokiana through the hyphae of the mycorrhizal fungi. To our knowledge, this is the second study to suggest the involvement of Ceratobasidiaceae fungi in tripartite symbiosis with achlorophyllous orchids and photosynthetic host plants.

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Acknowledgments

We thank Dr. Toshimitsu Fukiharu, Natural History Museum and Institute, Chiba, Mr. Noritoshi Inagaki, Kochi Prefectural Makino Botanical Garden, and Mr. Kazuo Mori, plant fancier in Hyogo Prefecture for their kind help in C. sikokiana sampling. We also thank Ms. Shiho Ikeda for technical assistance in DNA analyses.

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Authors and Affiliations

  1. Orchid Museum Takamori, 512-73 Izuhara, Takamori, Shimoina, Nagano, 399-3107, Japan

    Takahiro Yagame

  2. Biological Environment Institute, Research and Development Department, The General Environmental Technos, 8-4 Ujimatafuri, Uji, Kyoto, 611-0021, Japan

    Masahide Yamato

  3. Department of Biology, Faculty of Education, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan

    Akira Suzuki

  4. Fungus/Mushroom Resource and Research Center Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori, 680-8553, Japan

    Koji Iwase

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  1. Takahiro Yagame
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  2. Masahide Yamato
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  3. Akira Suzuki
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  4. Koji Iwase
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Correspondence to Takahiro Yagame.

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Yagame, T., Yamato, M., Suzuki, A. et al. Ceratobasidiaceae mycorrhizal fungi isolated from nonphotosynthetic orchid Chamaegastrodia sikokiana . Mycorrhiza 18, 97–101 (2008). https://doi.org/10.1007/s00572-007-0155-0

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