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Ultrastructural, fluorescence microscopic and microfluorimetric study of the innervation of the axial complex in the sea urchin, Sphaerechinus granularis (Lam.)

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Summary

In juxtaposition with the contractile epithelia of the axial complex of the sea urchin, Sphaerechinus granularis, several types of nerve fibers with different vesicle populations were determined. Nerve terminals, filled with clear vesicles and dense core vesicles, form synaptoid neuromuscular junctions. Close to the somatocoelic epithelia of the axial and terminal sinus septa, numerous axon profiles form a nerve plexus. Among the epithelial cells covering the plexus, two types of nerve cells can be distinguished which presumably produce neurosecretory and aminergic granules, respectively. Monoamine fluorescence (formaldehyde-induced fluorescence, Falck-Hillarp technique) was analyzed microspectrofluorimetrically. The emission spectrum of the fluorophores occurring in the present material shows a maximum at 475 nm and is characteristic of catecholamines; the excitation maximum at 380 nm after formaldehyde treatment is typical of catecholamines at low pH only. Since the peak ratio (370:320 nm) does not change after HCl-vapor treatment, the fluorophores are likely to be indicative of dopamine.

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References

  • Bachmann, S., Goldschmid, A.: Fine structure of the axial complex of Sphaerechinus granularis (Lam.) (Echinodermata/Echinoidea). Cell Tiss. Res. (1978)

  • Bargmann, W., Behrens, B.: Über den Feinbau des Nervensystems des Seesterns (Asterias rubens L.). II. Mitteilung zur Frage des Baues und der Innervation der Ampullen. Z. Zellforsch. 59, 746–770 (1963)

    Google Scholar 

  • Bargmann, W., Behrens, B.: Über die Pylorusanhänge des Seesterns (Asterias rubens L.), insbesondere ihre Innervation. Z. Zellforsch. 84, 536–584 (1968)

    Google Scholar 

  • Bargmann, W., Hehn, G. von: Über das Axialorgan (“mysterious gland”) von Asterias rubens L. Z. Zellforsch. 88, 262–277 (1968)

    Google Scholar 

  • Bargmann, W., Lindner, E., Andres, K.H.: Über die Synapsen an endokrinen Epithelzellen und die Definition sekretorischer Neurone. Untersuchungen am Zwischenlappen der Katzenhypophyse. Z. Zellforsch. 77, 282–298 (1967)

    Google Scholar 

  • Baumgarten, H.G.: Biogenic monoamines in the cyclostome and lower vertebrate brain. Progr. Histochem. Cytochem. 4, 1–90 (1972)

    Google Scholar 

  • Björklund, A., Falck, B., Owman, Ch.: Fluorescence microscopic and microspectrofluorometric techniques for the cellular localization and characterization of biogenic amines. In: Methods of investigative and diagnostic endocrinology (S.A. Berson, ed.), Vol. 1, The thyroid and biogenic amines (J.E. Rall, I.J. Kopin, eds.), pp. 318–368. Amsterdam: North-Holland Publ. Comp. 1972

    Google Scholar 

  • Boolootian, R.A., Campbell, J.L.: A primitive heart in the echinoid Strongylocentrotus purpuratus. Science 145, 173–175 (1964)

    Google Scholar 

  • Calas, A., Hartwig, H.-G., Collin, J.P.: Noradrenergic innervation of the median eminence. Micro-spectrofluorometric and pharmacological study in the duck, Anas platyrhynchos. Cell Tiss. Res. 147, 491–504 (1974)

    Google Scholar 

  • Cobb, J.L.S.: The distribution of monoamines in the nervous system of echinoderms. Comp. Biochem. Physiol. 28, 967–971 (1969)

    Google Scholar 

  • Cobb, J.L.S.: The innervation of the oesophagus of the sea urchin Heliocidaris erythrogramma. Z. Zellforsch. 98, 323–332 (1969)

    Google Scholar 

  • Cobb, J.L.S.: An ultrastructural study of the dermal papulae of the starfish, Asterias rubens, with special reference to innervation of the muscles. Cell Tiss. Res. 187, 515–523 (1978)

    Google Scholar 

  • Cobb, J.L.S., Laverack, M.S.: Neuromuscular systems in echinoderms. Symp. Zool. Soc. Lond. 20, 25–51 (1967)

    Google Scholar 

  • Cobb, J.L.S., Pentreath, V.W.: Anatomical studies of simple invertebrate synapses using stage rotation electron microscopy and densitometry. Tissue and Cell 9, 125–135 (1977)

    Google Scholar 

  • Cobb, J.L.S., Sneddon, E.: An ultrastructural study of the gills of Echinus esculentus. Cell Tiss. Res. 182, 265–274 (1977)

    Google Scholar 

  • Coleman, R.: Ultrastructure of the tube foot wall of a regular echinoid, Diadema antillarum Philippi. Z. Zellforsch. 96, 162–172 (1969)

    Google Scholar 

  • Corrodi, H., Hillarp, N.Å.: Fluoreszenzmethoden zur histochemischen Sichtbarmachung von Monoaminen. 2. Identifizierung des fluoreszierenden Produktes aus Dopamin und Formaldehyd. Helv. chim. Acta 47, 911–918 (1964)

    Google Scholar 

  • Cottrell, G.A., Pentreath, V.W.: Localization of catecholamines in the nervous system of a starfish, Asterias rubens, and of a brittle star, Ophiothrix fragilis. Comp. gen. Pharmacol. 1, 73–81 (1970)

    Google Scholar 

  • Dolder, H.: An ultrastructural and cytochemical study of neuromuscular junctions in echinoderms. Histochem. 44, 313–322 (1975)

    Google Scholar 

  • Elofsson, R., Falck, B., Lindvall, O., Myhrberg, H.: Evidence for new catecholamines in some invertebrate sensory neurons. Cell Tiss. Res. 182, 525–536 (1977)

    Google Scholar 

  • Falck, B., Hillarp, N.Å., Thieme, G., Torp, A.: Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10, 348–354 (1962)

    Google Scholar 

  • Falck, B., Owman, C.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. Lund., Sectio II, 7, 1–23 (1965)

    Google Scholar 

  • Florey, E., Cahill, M.A.: Ultrastructure of the sea urchin tube feet. Cell Tiss. Res. 177, 195–214 (1977)

    Google Scholar 

  • Florey, E., Cahill, M.A., Rathmayer, M.: Excitatory actions of GABA and of acetylcholine in sea urchin tube feet. Comp. Biochem. Physiol. 51, (C), 5–12 (1975)

    Google Scholar 

  • Hartwig, H.G.: Neurobiologische Studien an photoneuroendokrinen Systemen. Habilitationsschrift, Fachbereich Humanmedizin, Justus-Liebig-Universität Giessen, 1975

  • Hehn, G. von: Über den Feinbau des hyponeuralen Nervensystems des Seesternes (Asterias rubens L.). Z. Zellforsch. 105, 137–154 (1970)

    Google Scholar 

  • Holland, N.D.: The fine structure of the axial organ of the feather star, Nemaster rubiginosa (Echinodermata: Crinoidea). Tissue and Cell 2, 625–636 (1970)

    Google Scholar 

  • Jangoux, M., Schaltin, P.: Le complexe axial de Psammechinus miliaris (Gmelin) (Echinodermata, Echinoidea). Arch. Zool. exp. gén. 118, 285–303 (1977)

    Google Scholar 

  • Jonsson, G.: Fluorescence studies on some 6,7-substituted 3,4-dihydroisoquinolines formed from 3-hydroxytyramine (dopamine) and formaldehyde. Acta chem. scand. 20, 2755–2762 (1966)

    Google Scholar 

  • Juillard, M.T., Hartwig, H.-G., Collin, J.P.: The avian pineal organ. Distribution of endogenous monoamines; a fluorescence microscopic, microspectrofluorimetric and pharmacological study in the parakeet. J. Neural Transmission 40, 269–287 (1977)

    Google Scholar 

  • Leclerc, M.: L'organe axial et ses relations avec la sexualité et l'immunité chez les Astérides. Ann. Sci. Nat. Zool. 12e sér., 16, 285–359 (1974)

    Google Scholar 

  • Luft, J.H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)

    Google Scholar 

  • Pentreath, V.W., Cobb, J.L.S.: Neurobiology of Echinodermata. Biol. Rev. 47, 369–392 (1972)

    Google Scholar 

  • Pentreath, V.W., Cottrell, G.A.: Acetylcholine and cholinesterase in the radial nerve of Asterias rubens. Comp. Biochem. Physiol. 27, 775–785 (1968)

    Google Scholar 

  • Pentreath, V.W., Cottrell, G.A.: “Giant” neurons and neurosecretion in the hyponeural tissue of Ophiothrix fragilis Abildgaard. J. exp. mar. Biol. Ecol. 6, 249–264 (1971)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Rieder, E.: Untersuchungen über das Axialorgan der Echinoidea. Phil. Diss. Univ. Vienna, Zool. Inst. 1975

  • Rosenbluth, J.: Myoneural junctions of two ultrastructurally distinct types in earthworm bodywall muscles. J. Cell Biol. 54, 566–579 (1972)

    Google Scholar 

  • Simonsberger, P., Slattenscheck, W., Adam, H.: Eine digital gesteuerte Stickstoff-Spar-Schaltung für Gefriertrocknungsanlagen. Mikroskopie 33, 203–208 (1977)

    Google Scholar 

  • Tsuchiya, T., Amemiya, S.: Studies on the radial muscle of an echinothurid sea urchin Asthenosoma. I. Mechanical responses to electrical stimulation and drugs. Comp. Biochem. Physiol. 57 C, 69–73 (1977)

    Google Scholar 

  • Weber, W., Grosmann, M.: Ultrastructure of the basiepithelial nerve plexus of the sea urchin, Centrostephanus longispinus. Cell Tiss. Res. 175, 551–562 (1977)

    Google Scholar 

  • Welsch, U.: Catecholamine und 5-Hydroxytryptamin im Nervensystem von Seesternen und Enteropneusten. Z. Zellforsch. 115, 88–93 (1971)

    Google Scholar 

  • Welsh, J.H.: Neurohumours and neurosecretion. In: Physiology of Echinodermata (ed.: R.A. Boolootian), pp. 545–560. New York: Interscience 1966

    Google Scholar 

  • Welsh, J.H.: Catecholamines in the invertebrates. In: Handbook of experimental pharmacology, Vol. XXXIII, catecholamines. (H. Blaschko, E. Muscholl, eds.), pp. 79–109. Berlin-Heidelberg-New York: Springer 1972

    Google Scholar 

  • Wood, J.G.: Electron microscopic localization of amines in central nervous tissue. Nature (Lond.) 209, 1131–1133 (1966)

    Google Scholar 

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  1. Department of Zoology, University of Salzburg, Salzburg, Austria

    Sebastian Bachmann & Alfred Goldschmid

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  1. Sebastian Bachmann
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  2. Alfred Goldschmid
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Bachmann, S., Goldschmid, A. Ultrastructural, fluorescence microscopic and microfluorimetric study of the innervation of the axial complex in the sea urchin, Sphaerechinus granularis (Lam.). Cell Tissue Res. 194, 315–326 (1978). https://doi.org/10.1007/BF00220398

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