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Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris

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Immunocytochemistry and Related Techniques

Part of the book series: Neuromethods ((NM,volume 101))

Abstract

Here we present two protocols developed to investigate the spatial distribution and relationship of neuroactive substances in the nervous tissues of cephalopod molluscs . The protocols are designed for frozen and vibratome sections of the Octopus vulgaris brain , but are easily transferable to other cephalopod species and tissues, and are also specifically designed to detect small molecules such as monoamines . One of the two protocols has been adjusted to process paraformaldehyde -fixed tissues, while the second is designed for tissues that require glutaraldehyde fixation.

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Authors and Affiliations

  1. Associazione Cephalopod Research “CephRes”, Naples, Italy

    Giovanna Ponte

  2. Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Naples, Italy

    Graziano Fiorito

Authors
  1. Giovanna Ponte
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  2. Graziano Fiorito
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Correspondence to Graziano Fiorito .

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Editors and Affiliations

  1. Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Torino, Italy

    Adalberto Merighi

  2. Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Torino, Italy

    Laura Lossi

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Ponte, G., Fiorito, G. (2015). Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris . In: Merighi, A., Lossi, L. (eds) Immunocytochemistry and Related Techniques. Neuromethods, vol 101. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2313-7_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2313-7_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2312-0

  • Online ISBN: 978-1-4939-2313-7

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