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An effective method of sonication-assisted Agrobacterium-mediated transformation of chickpeas

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Abstract

An efficient and reproducible transformation method of sonication- assisted Agrobacterium-mediated transformation (SAAT) was developed for chickpea (Cicer arietinum L.). Agrobacterium tumefaciens (LBA4404) harboring pCAMBIA1305.2 was used to transform decapitated embryo explants of two cultivars of chickpeas. By using a series of co-cultivation, callus induction, shoot initiation and root inducing media, a large number of transgenic plants were recovered. Transient expressions of GUS gene were detected by X-Gluc histochemical assay in transformed tissues. DNA analysis of T0 and T1 plants by PCR and Southern hybridization confirmed the integration of transgenes in initial and next generation transformants in different transgenic lines. The transformation efficiency was more than two times higher in SAAT treatment than simple Agrobacterium without sonication.

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Acknowledgements

The work was supported by Dr. Basma Al-Baharna, Director, Biotechnology Program and supporting grants from the Biotechnology Program of Arabian Gulf University, Manama, Kingdom of Bahrain.

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  1. Biotechnology Program, Arabian Gulf University, P. O. Box 26671, Manama, Kingdom of Bahrain

    Malabika Roy Pathak & Riyad Yousif Hamzah

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  1. Malabika Roy Pathak
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  2. Riyad Yousif Hamzah
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Correspondence to Malabika Roy Pathak.

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Pathak, M.R., Hamzah, R.Y. An effective method of sonication-assisted Agrobacterium-mediated transformation of chickpeas. Plant Cell Tiss Organ Cult 93, 65–71 (2008). https://doi.org/10.1007/s11240-008-9344-6

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  • DOI: https://doi.org/10.1007/s11240-008-9344-6

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