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Agrobacterium-mediated transformation of chickpea with α-amylase inhibitor gene for insect resistance

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Abstract

Chickpea is the world’s third most important pulse crop and India produces 75% of the world’s supply. Chickpea seeds are attacked byCallosobruchus maculatus andC. chinensis which cause extensive damage. The α-amylase inhibitor gene isolated fromPhaseolus vulgaris seeds was introduced into chickpea cultivar K850 throughAgrobacterium- mediated transformation. A total of 288 kanamycin resistant plants were regenerated. Only 0.3% of these were true transformants. Polymerase chain reaction (PCR) analysis and Southern hybridization confirmed the presence of 4.9 kb α-amylase inhibitor gene in the transformed plants. Western blot confirmed the presence of α-amylase inhibitor protein. The results of bioassay study revealed a significant reduction in the survival rate of bruchid weevilC. maculatus reared on transgenic chickpea seeds. All the transgenic plants exhibited a segregation ratio of 3:1.

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  1. Entomology Research Institute, Loyola College, 600 034, Chennai, India

    S. Ignacimuthu & S. Prakash

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  1. S. Ignacimuthu
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  2. S. Prakash
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Correspondence to S. Ignacimuthu.

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Ignacimuthu, S., Prakash, S. Agrobacterium-mediated transformation of chickpea with α-amylase inhibitor gene for insect resistance. J. Biosci. 31, 339–345 (2006). https://doi.org/10.1007/BF02704106

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  • DOI: https://doi.org/10.1007/BF02704106

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