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Characterization of an anther- and tapetum-specific gene and its highly specific promoter isolated from tomato

  • Physiology and Biochemistry
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An Erratum to this article was published on 17 February 2009

Abstract

A full-length genomic clone of 2,233 bp long containing an anther- and tapetum-specific gene TomA108 was isolated and characterized from tomato. The gene was present in one copy per haploid genome. The isolated clone contained 5′ and 3′ untranslated regions of 810 and 170 nucleotides, respectively and a single intron with highly repetitive sequences. The cDNA encoded the protein with an apparent mass of 10.6 kDa and a pI (isoelectric point) of 5.3. It was cysteine-rich and had an N-terminal hydrophobic domain with characteristics of a secretory signal. Amino acid sequence comparisons demonstrated that the protein was closely related to a family of cereal seed storage proteins and protease inhibitors. The fusion of β-glucuronidase to the TomA108 promoter demonstrated that the promoter was highly active from early-meiosis to free microspores production in tapetum of tobacco. This strong and highly specific promoter can be potentially used to generate male sterility for efficient production of plant hybrids.

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Abbreviations

GUS:

β-Glucuronidase

EDTA:

Ethylene diamine tetraacetie acid

MADS:

MCM1, AGAMOUS, DEFICIENS, and SRF

CarG:

(CC(A/T)6GG)

GA:

Gibbrerllin

ABA:

Abscisic acid

SA:

Salicylic acid

SDS:

Sodium dodecyl sulfate

IPTG:

Isopropyl-1-thio-β-d-alactoside

PAGE:

Polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China

    S. X. Xu & G. S. Liu

  2. Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada

    R. D. Chen

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  1. S. X. Xu
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  2. G. S. Liu
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  3. R. D. Chen
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Correspondence to G. S. Liu.

Additional information

Communicated by Y. Lu

An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-009-0682-6.

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Xu, S.X., Liu, G.S. & Chen, R.D. Characterization of an anther- and tapetum-specific gene and its highly specific promoter isolated from tomato. Plant Cell Rep 25, 231–240 (2006). https://doi.org/10.1007/s00299-005-0056-7

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