Abstract
Nuclear male sterility within Triticum aestivum is considered as the ideal basis for the development of a hybridization system for wheat. We engineered nuclear male sterility in wheat by introducing the barnase gene under the control of tapetum-specific promoters derived from corn and rice. A biolistic-mediated transformation method, based on the use of the poly(ADP-ribose)polymerase inhibitor niacinamide, was set up which enriched for low-copy integrations (1–3 copies). Most of these copies were not linked and segregated in the next generation.
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Received: 22 January 1997 / 7 February 1997
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Block, M., Debrouwer, D. & Moens, T. The development of a nuclear male sterility system in wheat. Expression of the barnase gene under the control of tapetum specific promoters. Theor Appl Genet 95, 125–131 (1997). https://doi.org/10.1007/s001220050540
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DOI: https://doi.org/10.1007/s001220050540