Abstract
The leaf disc transformation/regeneration system was modified for tomato (L. esculentum). Both leaf explants and cotyledon/hypocotyl sections can be used to regenerate transformed plants. We have obtained over 300 transgenic plants from eight tomato cultivars. We have evidence for both single and multi-copy insertions of the T-DNA, and have demonstrated inheritance of the T-DNA insert in the expected Mendelian ratios. Several heterologous promoters function in tomato. A reduced efficiency of transformation was observed with binary T-DNA vectors as compared to co-integrate T-DNA vectors. The ease of the leaf disc method makes tomato a premier experimental organism for plant biotechnology.
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Abbreviations
- BA:
-
benzyl adenine
- IAA:
-
indole acetic acid
- LB:
-
Luria Broth
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Communicated by I.K. Vasil
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McCormick, S., Niedermeyer, J., Fry, J. et al. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens . Plant Cell Reports 5, 81–84 (1986). https://doi.org/10.1007/BF00269239
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DOI: https://doi.org/10.1007/BF00269239