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A genetic linkage map of Brassica carinata constructed with a doubled haploid population

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Abstract

Brassica carinata is an important oilseed crop with unique favourable traits that are desirable for other Brassica crops. However, given the limited research into genetic resources in B. carinata, knowledge of the genetic structure of this species is relatively poor. Nine homozygous, genetically distinct accessions of B. carinata were obtained via microspore culture, from which two divergent doubled haploid (DH) lines were used to develop a DH mapping population that consisted of 183 lines. The mapping population showed segregation of multiple traits of interest. A genetic map was constructed with PCR-based markers, and a total of 212 loci, which covered 1,703 cM, were assigned to eight linkage groups in the B genome and nine linkage groups in the C genome, which allowed comparison with genetic maps of other important Brassica species that contain the B/C genome(s). Loci for two Mendelian-inherited traits related to pigmentation (petal and anther tip colour) and one quantitative trait (seed coat colour) were identified using the linkage map. The significance of the mapping population in the context of genetic improvement of Brassica crops is discussed.

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Acknowledgments

This study was supported by the National Science Foundation of China (Project code: 30830073). Jun Zou was supported by the China Postdoctoral Science Foundation (20100471198).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Shaomin Guo, Jun Zou, Ruiyan Li, Yan Long & Jinling Meng

  2. School of Plant Biology and The UWA Institute of Agriculture, The University of Western Australia, Crawley, WA, 6009, Australia

    Sheng Chen

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  1. Shaomin Guo
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  2. Jun Zou
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  3. Ruiyan Li
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  4. Yan Long
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  5. Sheng Chen
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  6. Jinling Meng
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Correspondence to Jinling Meng.

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Communicated by A. Bervillé.

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Guo, S., Zou, J., Li, R. et al. A genetic linkage map of Brassica carinata constructed with a doubled haploid population. Theor Appl Genet 125, 1113–1124 (2012). https://doi.org/10.1007/s00122-012-1898-3

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