Skip to main content
SpringerLink
Log in

Mapping of yield influencing QTL in Brassica juncea: implications for breeding of a major oilseed crop of dryland areas

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Quantitative trait loci (QTL) analysis of yield influencing traits was carried out in Brassica juncea (AABB) using a doubled haploid (DH) mapping population of 123 lines derived from a cross between Varuna (a line representing the Indian gene pool) and Heera (representing the east European gene pool) to identify potentially useful alleles from both the parents. The existing AFLP based map of B. juncea was further saturated with RFLP and SSR markers which led to the identification of the linkage groups belonging to the A (B. rapa) and B (B. nigra) genome components of B. juncea. For QTL dissection, the DH lines were evaluated at three different environments and phenotyped for 12 quantitative traits. A total of 65 QTL spread over 13 linkage groups (LG) were identified from the three environments. QTL analysis showed that the A genome has contributed more than the B genome to productivity (68% of the total QTL detected) suggesting a more prominent role of the A genome towards domestication of this crop. The east European line, Heera, carried favorable alleles for 42% of the detected QTL and the remaining 58% were in the Indian gene pool line, Varuna. We observed clustering of major QTL in a few linkage groups, particularly in J7 and J10 of the A genome, with QTL of different traits having agronomically antagonistic allelic effects co-mapping to the same genetic interval. QTL analysis also identified some well-separated QTL which could be readily transferred between the two pools. Based on the QTL analysis, we propose that improvement in yield could be achieved more readily by heterosis breeding rather than by pure line breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Asins MJ (2002) Present and future of quantitative trait locus analysis in plant breeding. Plant Breed 121:281–291

    Article  Google Scholar 

  • Butruille DV, Guries RP, Osborn TC (1999) Linkage analysis of molecular markers and quantitative traits loci in populations of inbred backcross lines of Brassica napus L. Genetics 153:949–964

    PubMed  CAS  Google Scholar 

  • Burns MJ, Barnes SR, Bowman JG, Clarke MHE, Werner CP, Kearsey MJ (2003) QTL analysis of an intervarietal set of substitution lines in Brassica napus: seed oil content and fatty acid composition. Heredity 90:39–48

    Article  PubMed  CAS  Google Scholar 

  • Cheung WY, Landry BS, Raney P, Rakow GFW (1997) Molecular mapping of seed quality traits in Brassica juncea L. Czern & Coss. In: Proceedings of international symposium on Brassica. Acta Hort 459:139–147

  • Churchill GA, Doerge RW (1994) Emperical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Deorge RW, Churchill GA (1996) Permutation test for multiple loci affecting a quantitative character. Genetics 142:285–294

    Google Scholar 

  • Ecke W, Uzunova M, Weissleder K (1995) Mapping the genome of rapeseed (Brassica napus L.) II. Localization of genes controlling erucic acid synthesis and seed oil content. Theor Appl Genet 91:972–977

    Article  CAS  Google Scholar 

  • Gupta V, Mukhopadhyay A, Arumugam N, Sodhi YS, Pental D, Pradhan AK (2004) Molecular tagging of erucic acid trait in oilseed mustard (Brassica juncea) by QTL mapping and single nucleotide polymorphisms in FAE1 gene. Theor Appl Genet 108:743–749

    Article  PubMed  CAS  Google Scholar 

  • Jagannath A, Bandyopadhyay P, Arumugam N, Gupta V, Burma PK, Pental D (2001) The use of Spacer DNA fragment insulates the tissue specific expression of a cytotoxic gene (barnase) and allows high frequency generation of transgenic male sterile lines in Brassica juncea L. Mol Breed 8:11–23

    Article  CAS  Google Scholar 

  • Jagannath A, Arumugam N, Gupta V, Pradhan AK, Burma PK, Pental D (2002) Development of transgenic barstar lines and identification of a male sterile (barnase)/restorer(barstar) combination for heterosis breeding in Indian oilseed mustard (Brassica juncea). Curr Sci 82:46–52

    CAS  Google Scholar 

  • Mika V, Tillmann P, Kopma R, Nerusil P, Kucera V (2003) Fast prediction of quality parameters in whole seeds of oilseed rape (Brassica napus L.). Plant Soil Environ 49:141–145

    Google Scholar 

  • Padmaja KL, Arumugam N, Gupta V, Mukhopadhyay A, Sodhi YS, Pental D, Pradhan AK (2005) Mapping and tagging of seed coat colour and the identification of microsatellite markers for marker-assisted manipulation of the trait in Brassica juncea. Theor Appl Genet 111:8–14

    Article  PubMed  CAS  Google Scholar 

  • Parkin IAP, Sharpe AG, Keith DJ, Lydiate DJ (1995) Identification of the A and C genomes of amphidiploid Brassica napus (oilseed rape). Genome 38:1122–1131

    CAS  PubMed  Google Scholar 

  • Piquemal J, Cinquin E ,Couton F, Rondean C, Seignoret E, Doucet I, Perret D, Villeger MJ, Vincourt P, Blanchard P (2005) Constructiion of an oilseed rape (Brassica napus L) genetic map with SSR markers. Theor Appl Genet 111:1514–1523

    Article  PubMed  CAS  Google Scholar 

  • Pradhan AK, Sodhi YS, Mukhopadhyay A, Pental D (1993) Heterosis breeding in Indian mustard (Brassica juncea L. Czern & Cross): analysis of component characters contributing to heterosis for yield. Euphytica 69:219–229

    Article  Google Scholar 

  • Pradhan AK, Gupta V, Mukhopadhyay A, Arumugam N, Sodhi YS, Pental D (2003) A high density linkage map in Brassica juncea (Indian mustard) using AFLP and RFLP markers. Theor Appl Genet 106:607–614

    PubMed  CAS  Google Scholar 

  • Qiu D, Morgan C, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I (2006) A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theor Appl Genet 114:67–80

    Article  PubMed  CAS  Google Scholar 

  • Quijada PA, Udall JA, Lambert B, Osborn TC (2006) Quantitative trait analysis of seed yield and other complex traits in hybrid spring rapeseed (Brassica napus L.): 1. Identification of genomic regions from winter germplasm. Theor Appl Genet 113:549–561

    Article  PubMed  CAS  Google Scholar 

  • Ragot M, Sisco PH, Hoisington DA, Stuber CW (1995) Molecular-marker-mediated characterization of favourable exotic alleles at quantitative trait loci in maize. Crop Sci 35:1306–1315

    Article  CAS  Google Scholar 

  • Rogers SO, Bendich AJ (1994) Extraction of total cellular DNA from plants, algae and fungi. In: Gelvin SV, Shilperoot RA (eds) Plant molecular biology manual. Kluwer, Dordrecht, pp 1–8

    Google Scholar 

  • Sharpe AG, Parkin lAP, Keith DJ, Lydiate DJ (1995) Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). Genome 38:1112–1121

    CAS  PubMed  Google Scholar 

  • Sodhi YS, Chandra A, Verma JK, Arumugam N, Mukhopadhyay A, Gupta V, Pental D, Pradhan AK (2006) A new cytoplasmic male sterility system for hybrid seed production in Indian oilseed mustard Brassica juncea. Theor Appl Genet 114:93–99

    Article  PubMed  CAS  Google Scholar 

  • Song K, Osborn TC, William PH (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLP): I. Genome evolution of diploid and amphidiploid species. Theor Appl Genet 75:784–794

    Article  CAS  Google Scholar 

  • SPAR 1 (Statistical package for agricultural research data analysis) (1991) Indian Agricultural Statistical Research Institute, New Delhi

  • Srivastava A, Gupta V, Pental D, Pradhan AK (2001) AFLP-based genetic diversity assessment amongst agronomically important natural and some newly synthesized lines of Brassica juncea. Theor Appl Genet 102:193–199

    Article  CAS  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Stam P, Van Ooijen JW (1996) Joinmap version 2.0: software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen

    Google Scholar 

  • Tanksley SD, Grandillo S, Fulton TM, Zamir D, Eshed Y, Petiard V, Lopez J, Beck-Bunn T (1996) Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium. Theor Appl Genet 92:213–224

    Article  CAS  Google Scholar 

  • Teutenico RA, Osborn TC (1994) Mapping of RFLP and qualitative trait loci in Brassica rapa and comparison to the linkage maps of B. napus, B. olecracea ,and Arabidosis thaliana. Theor Appl Genet 89:885–894

    Google Scholar 

  • Udall JA, Quijada PA , Lambert B, Osborn TC (2006) Quantitative trait analysis of seed yield and other complex traits in hybrid spring rapeseed (Brassica napus L.): 2. Identification of alleles from unadapted germplasm. Theor Appl Genet 113:597–609

    Article  PubMed  CAS  Google Scholar 

  • Wang S, Basten CJ, Gaffney P, Zeng ZB (2001–2005) Windows QTL Cartographer version 2.5. North Carolina State University, Bioinformatics Research Center, Raleigh

  • Xiao J, Li J, Grandillo S, Ahn SN, Yuan L, Tanksley SD, McCouch SR (1998) Identification of trait improving quantitative trait loci alleles from a wild rice relative Oryza rufipogon. Genetics 150:899–909

    PubMed  CAS  Google Scholar 

  • Zeng ZB (1993) Theoretical basis for separation of multiple linked gene effects in mapping quantitative trait loci. Proc Natl Acad Sci USA 90:10972–10976

    Article  PubMed  CAS  Google Scholar 

  • Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Dhara Vegetable Oil and Food Company Ltd (DOFCO), a fully owned company of the National Dairy Development Board (NDDB) and the Department of Biotechnology (DBT). N. Ramchiary acknowledges the receipt of a fellowship from the Council of Scientific and Industrial Research (CSIR), Government of India.

Author information

Author notes

  1. N. Arumugam

    Present address: Department of Biotechnology, Pondicherry University, Pondicherry, 605014, India

Authors and Affiliations

  1. Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    V. Gupta, Y. S. Sodhi, A. Mukhopadhyay, N. Arumugam, D. Pental & A. K. Pradhan

  2. Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    N. Ramchiary, K. L. Padmaja, S. Sharma, D. Pental & A. K. Pradhan

Authors
  1. N. Ramchiary
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. L. Padmaja
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. S. Sodhi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Mukhopadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. N. Arumugam
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. D. Pental
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. K. Pradhan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. K. Pradhan.

Additional information

Communicated by F. van Eeuwijk.

Electronic supplementary material

Below is the link to the electronic supplementary material.

(PPT 727 kb)

(PPT 622 kb)

(DOC 100 kb)

(DOC 239 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ramchiary, N., Padmaja, K.L., Sharma, S. et al. Mapping of yield influencing QTL in Brassica juncea: implications for breeding of a major oilseed crop of dryland areas. Theor Appl Genet 115, 807–817 (2007). https://doi.org/10.1007/s00122-007-0610-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-007-0610-5

Keywords

Search

Navigation