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Antioxidants and stress-related metabolites in the seedlings of two indica rice varieties exposed to cadmium chloride toxicity

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Abstract

We present here a comprehensive study depicting the differences in biochemical responses to increasing CdCl2 concentrations (0.1, 0.25, 0.5 and 1.5 mM) in the two indica rice varieties, IR-29 (salt-sensitive) and Nonabokra (salt-tolerant), in order to contribute to our understanding of genotypic variation of cadmium tolerance. The oxidative damages in both the varieties enhanced with the increase in CdCl2 concentrations, the susceptibility of IR-29 being more pronounced than Nonabokra. The detrimental effects in IR-29 were reflected in greater chlorophyll loss, higher H2O2 and malondialdehyde content even at lower concentrations and drastically higher lipoxygenase activity, protein oxidation and putrescine accumulation, especially at higher CdCl2 levels. The antioxidants like anthocyanin and carotenoids, antioxidative enzymes like guaiacol peroxidase (GPX) and ascorbate peroxidase (APX), osmolytes like proline, reducing sugars, spermidine and spermine, increased in both the varieties with CdCl2 levels. While anthocyanin, reducing sugars and spermine showed greater increment in IR-29, the GPX/APX activity was more enhanced in Nonabokra; the increase in carotenoids, proline and spermidine being similar in both the varieties. However, reverse trends were noted for cysteine level and CAT activity; IR-29 showed marked decrease in cysteine content and CAT activity with increased cadmium exposure, whereas in Nonabokra, both the parameters increased, particularly at higher cadmium levels. Thus, the detoxification mechanism in the more-susceptible IR-29 was probably rendered by anthocyanin, reducing sugars and spermine in particular, as well as by GPX/APX, rather than cysteine and CAT, which showed cadmium sensitivity. Thus, the CdCl2 stress-dependent comparative biochemical analyses displayed major differences in the two rice varieties in terms of tolerance to Cd toxicity. Our data provides evidence that Nonabokra, which is a well-known variety tolerant to sodium chloride toxicity, also shows promising tolerance to cadmium toxicity, and hints at their possible utilization in Cd remediation.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

Cys:

Cysteine

GPX:

Guaiacol peroxidase

LOX:

Lipoxygenase

MDA:

Malondialdehyde

PAs:

Polyamines

Pro:

Proline

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

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Acknowledgments

Financial support from the Department of Biotechnology, Government of India, New Delhi as well as Bose Institute Research Fellowship is gratefully acknowledged.

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Authors and Affiliations

  1. Post Graduate Department of Biotechnology, St. Xavier’s College, 30, Mother Teresa Sarani, Park Street, Kolkata, 700016, West Bengal, India

    Aryadeep Roychoudhury

  2. Division of Plant Biology, Bose Institute (Main Campus), 93/1, A. P. C. Road, Kolkata, 700009, West Bengal, India

    Supratim Basu & Dibyendu N. Sengupta

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  1. Aryadeep Roychoudhury
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  2. Supratim Basu
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  3. Dibyendu N. Sengupta
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Correspondence to Aryadeep Roychoudhury.

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Communicated by G. Klobus.

A. Roychoudhury and S. Basu have equal contributions.

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Roychoudhury, A., Basu, S. & Sengupta, D.N. Antioxidants and stress-related metabolites in the seedlings of two indica rice varieties exposed to cadmium chloride toxicity. Acta Physiol Plant 34, 835–847 (2012). https://doi.org/10.1007/s11738-011-0881-y

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