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Overexpression of cinnamyl alcohol dehydrogenase gene from sweetpotato enhances oxidative stress tolerance in transgenic Arabidopsis

  • Genetic Transformation
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Abstract

Cinnamyl alcohol dehydrogenase (CAD) is the enzyme in the last step of lignin biosynthetic pathway and is involved in the generation of lignin monomers. IbCAD1 gene in sweetpotato (Ipomoea batatas) was identified, and its expression was induced by abiotic stresses based on promoter analysis. In this study, transgenic Arabidopsis plants overexpressing IbCAD1 directed by CaMV 35S promoter were developed to determine the physiological function of IbCAD1. IbCAD1-overexpressing transgenic plants exhibited better plant growth and higher biomass compared to wild type (WT), under normal growth conditions. CAD activity was increased in leaves and roots of transgenic plants. Sinapyl alcohol dehydrogenase activity was induced to a high level in roots, which suggests that IbCAD1 may regulate biosynthesis of syringyl-type (S) lignin. Lignin content was increased in stems and roots of transgenic plants; this increase was in S lignin rather than guaiacyl (G) lignin. Overexpression of IbCAD1 in Arabidopsis resulted in enhanced seed germination rates and tolerance to reactive oxygen species (ROS), such as hydrogen peroxide (H2O2). Taken together, our results show that IbCAD1 controls lignin content by biosynthesizing S units and plays an important role in plant responses to oxidative stress.

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Funding

This work was supported by the 2015 Inje University research grant.

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Authors and Affiliations

  1. Ubiquitous healthcare research center, Inje University, Gimhae, Gyeongsangnam-do, 50834, Republic of Korea

    Young-Hwa Kim & Gyung-Hye Huh

  2. Institute of Digital Anti-Aging Healthcare, Graduate School of Inje University, Gimhae, Gyeongsangnam-do, 50834, Republic of Korea

    Young-Hwa Kim & Gyung-Hye Huh

  3. Department of Healthcare Information Technology, Inje University, Gimhae, Gyeongsangnam-do, 50834, Republic of Korea

    Gyung-Hye Huh

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  1. Young-Hwa Kim
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  2. Gyung-Hye Huh
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Correspondence to Gyung-Hye Huh.

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Editor: Yong Eui Choi

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Kim, YH., Huh, GH. Overexpression of cinnamyl alcohol dehydrogenase gene from sweetpotato enhances oxidative stress tolerance in transgenic Arabidopsis. In Vitro Cell.Dev.Biol.-Plant 55, 172–179 (2019). https://doi.org/10.1007/s11627-018-09951-5

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  • DOI: https://doi.org/10.1007/s11627-018-09951-5

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