Abstract
Vibrio splendidus is a ubiquitous pathogen that causes various diseases in aquaculture with a wide range of hosts. In our previous studies, we showed that l-glutamic acid was the optimal carbon source that could revive V. splendidus persister cells. In our present study, single cell observation under microscopy showed that V. splendidus could revive using l-glutamic acid as carbon source. A proteomic analysis was carried out to further illustrate the initial wake up of persister cells with l-glutamic acid. To collect the initially revived cells, SDS-PAGE was used to determine the revived time. The total proteins from the persister cells and the revived cells were analyzed using LC‒MS/MS. A total of 106 proteins, including 42 downregulated proteins and 64 upregulated proteins, were identified. GO analysis of the differentially expressed proteins (DEPs) showed that biological processes, including protein complex assembly, protein oligomerization, and arginine metabolism; cellular components, including extracellular membrane, plasma membrane and ribosome; and molecular functions, including the activities of arginine binding and structural constituent of ribosome, were enriched. KEGG analysis showed that lipopolysaccharide biosynthesis, porphyrin and chlorophyll metabolism, and peptidoglycan biosynthesis were upregulated, while the ribosome was downregulated. This is the first time to study the initial wake up of persister cells based on proteomic analysis, and the results revealed the main pathways involved in the early resuscitation of V. splendidus persister cells.
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Funding
This work was finally supported by the Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholar (LR20C190001), the National Natural Science Foundation of China (31972833), the Fundamental Research Funds for the Provincial Universities of Zhejiang (SJLZ2020001), and the K.C. Wong Magna Fund at Ningbo University.
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GJ: conducted the experiments, analyzed the data, and wrote the original manuscript. YL: conducted parts of the experiments and analyzed the data. JZ: conducted parts of the experiments. WL analyzed parts of the data. WD conceived and planned the research, and revised the manuscript. WZ: conceived and planned the research, supervised the research, revised the manuscript, and acquired funding.
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Jiang, G., Li, Y., Zhang, J. et al. Proteomic analysis of the initial wake up of vibrio splendidus persister cells. World J Microbiol Biotechnol 39, 116 (2023). https://doi.org/10.1007/s11274-023-03559-7
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DOI: https://doi.org/10.1007/s11274-023-03559-7