Abstract
Vibrio tubiashii (Vt) infection is a common cause of severe diseases in aquaculture. In order to confirm the pathogenicity of Vt in ivory snail Babylonia areolata, the Vt strains were analyzed using isobaric tags for relative and absolute quantitation (iTRAQ) technology. Thus, 2717 proteins were identified. Fifty-three upregulated proteins and 52 downregulated proteins were found in the high-virulence strain (BBXP), compared with the low-virulence strain (FB13). The results suggested that the membrane proteins, such as type I secretion outer membrane protein, TolC, lipoprotein, efflux system proteins, iron-regulated protein A, and several virulence-associated proteins, were differentially regulated. Analysis of the different proteins showed that many were associated with quorum sensing regulation, and that there were significant differences between the highest-virulence strain and the lowest-virulence strain, including differences in their transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis. Cell division proteins and cell repair proteins differed most significantly and the levels of these proteins in the virulent BBXP strain were much higher. The cell pathways related to stress responses and cell damage and the apoptosis pathways were significantly upregulated. This is the first study to identify differential proteins between Vt strains with different virulence levels. It will provide an important foundation for understanding the molecular mechanisms of the pathology of Vt in mollusks.
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Funding
This study was supported financially by grants from the National Natural Science Foundation of China (NSFC) (31902416), the Guangdong Provincial Key construction discipline research ability enhancement project (2021ZDJS041), and the Innovative school strengthening project of Guangdong Provincial Department of Education (2018KTSCX134).
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RX Wang designed the study. RX Wang and XJ Liu performed the study. RX Wang, T Zhang, JF Sun, XL Ma, and H Zhu developed the data analysis strategy. RX Wang, XJ Liu and JY Wang, XZ Lin, and HL Mou analyzed the data and assisted with the analytic tools. RX Wang wrote the manuscript. All the authors reviewed and approved the manuscript.
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Wang, R., Liu, X., Wang, J. et al. Proteomic differences between Vibrio tubiashii strains with high- or low-virulence levels isolated from diseased ivory snail Babylonia areolata. Aquacult Int 30, 2579–2591 (2022). https://doi.org/10.1007/s10499-022-00919-2
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DOI: https://doi.org/10.1007/s10499-022-00919-2