Skip to main content
SpringerLink
Log in

Somatic embryogenesis and plant regeneration from root sections of Allium schoenoprasum L.

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

A protocol has been developed for somatic embryogenesis and subsequent plant regeneration in Allium schoenoprasum L. Calli were induced from root sections isolated from axenic seedlings and cultivated on media containing either Murashige and Skoog’s (MS) or Dunstan and Short’s mineral solution supplemented with 5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 6-benzylaminopurine (BA), 6-furfurylaminopurine (Kin) or thidiazuron (TDZ) at 1, 5 or 10 μM. The highest frequencies of callus induction were achieved on media with 5 μM 2,4-D in combination with 5 μM TDZ or 10 μM BA (78.9% and 78.4%, respectively). Calli were then transferred to 1 μM 2,4-D, where compact yellow callus turned to segmented yellowish callus with transparent globular somatic embryos at the surface. Calli that were previously grown on media with 5 μM 2,4-D in combination with 10 μM BA or 10 μM TDZ showed the highest frequencies of embryogenic callus formation (45% and 42%) as well as mean number of somatic embryos per regenerating callus. The choice of mineral solution formulation did not significantly affect callus induction or embryogenic callus formation. The embryos could complete development into whole plants on plant growth regulator (PGR)-free medium, but inclusion of Kin (0.5, 2.5 and 5 μM) in this phase improved somatic embryo development and multiplication. Subsequently transferred to 1/2 MS PGR-free medium, all embryos rooted and the survival rate of the plants in a greenhouse was 96%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

ANOVA:

Analysis of variance

BA:

6-Benzylaminopurine

BDS:

Dunstan and Short’s mineral solution

2,4-D:

2,4-Dichlorophenoxyacetic acid

Kin:

6-Furfurylaminopurine

MS:

Murashige and Skoog’s mineral solution

PGR:

Plant growth regulator

TDZ:

Thidiazuron

References

  • Ayabe M, Sumi S (1998) Establishment of a novel tissue culture method, stem-disc culture, and its practical application to micropropagation of garlic (Allium sativum L.). Plant Cell Rep 17:773–779

    Article  CAS  Google Scholar 

  • Ayabe M, Sumi S (2001) A novel and efficient tissue culture method—“stem-disk dome culture”—for producing virus-free garlic (Allium sativum L.). Plant Cell Rep 20:503–507

    Article  CAS  Google Scholar 

  • Barandiaran X, Martín N, Rodríguez-Conde MF, Di Pietro A, Martín J (1999a) Genetic variability in callus formation and regeneration of garlic (Allium sativum L.). Plant Cell Rep 18:434–437

    Article  CAS  Google Scholar 

  • Barandiaran X, Martín N, Rodríguez-Conde MF, Di Pietro A, Martín J (1999b) An efficient method for callus culture and shoot regeneration of garlic (Allium sativum L.). Hort Sci 34:348–349

    Google Scholar 

  • Buiteveld J, Creemers-Molenaar J (1994) Plant regeneration from protoplasts isolated from suspension cultures of leek (Allium ampeloprasum L.). Plant Sci 100:203–210

    Article  CAS  Google Scholar 

  • Buiteveld J, Van der Valk P, Jansen J, Creemers-Molenaar J, Colijn-Hooymans CM (1993) Callus induction and plant regeneration from explants of commercial cultivars of leek (Allium ampeloprasum var. porrum L.). Plant Cell Rep 12:431–434

    Article  Google Scholar 

  • Debergh P, Standaert-De Metsenaere R (1976) Neoformation of bulbils in Allium porrum L. cultured in vitro. Sci Hort 5:11–12

    Article  Google Scholar 

  • Dunstan DI, Short KC (1977) Improved growth of tissue cultures of the onion, Allium cepa. Physiol Plant 41:70–72

    Article  Google Scholar 

  • Dunstan DI, Short KC (1978) Shoot production from onion callus tissue culture. Sci Hortic 9:99–110

    Article  Google Scholar 

  • Eady CC, Butler RC, Suo Y (1998) Somatic embryogenesis and plant regeneration from immature embryo cultures of onion (Allium cepa). Plant Cell Rep 18:111–116

    Article  CAS  Google Scholar 

  • Fereol L, Chovelon V, Causse S, Michaux-Ferriere N, Kahane R (2002) Evidence of a somatic embryogenesis process for plant regeneration in garlic (Allium sativum L.). Plant Cell Rep 21:197–203

    Article  CAS  Google Scholar 

  • Fereol L, Chovelon V, Causse S, Triarie D, Arnault I, Auger J, Kahane R (2005) Establishment of embryogenic cell suspension cultures of garlic (Allium sativum L.), plant regeneration and biochemical analyses. Plant Cell Rep 24:319–325

    Article  CAS  PubMed  Google Scholar 

  • Haque MS, Wada T, Hattori K (1997) High frequency shoot regeneration and plantlet formation from root tip of garlic. Plant Cell Tiss Org Cult 50:83–89

    Article  Google Scholar 

  • Hong W, Debergh P (1995) Somatic embryogenesis and plant regeneration in garden leek. Plant Cell Tiss Org Cult 43:21–28

    Article  Google Scholar 

  • Kim JW, Soh WY (1996) Plant regeneration through somatic embryogenesis from suspension cultures of Allium fistulosum L. Plant Sci 114:215–220

    Article  CAS  Google Scholar 

  • Luciani G, Marinangeli PA, Curvetto NR (2001) Increasing nitrate/ammonium ratio for improvement of garlic micropropagation. Sci Hort 87:11–20

    Article  CAS  Google Scholar 

  • Luciani GF, Mary AK, Pellegrini C, Curvetto NR (2006) Effects of explants and growth regulators in garlic callus formation and plant regeneration. Plant Cell Tiss Organ Cult 87:139–143

    Article  CAS  Google Scholar 

  • Luthar Z, Bohanec B (1999) Induction of direct somatic organogenesis in onion (Allium cepa L.) using a two-step flower or ovary culture. Plant Cell Rep 18:797–802

    Article  CAS  Google Scholar 

  • Martín-Urdíroz N, Garrido-Gala J, Martín J, Barandiaran X (2004) Effect of light on the organogenic ability of garlic using a one-step in vitro system. Plant Cell Rep 22:721–724

    Article  PubMed  Google Scholar 

  • Matsuda Y, Adachi T (1996) Plant regeneration via embryogenesis in commercial cultivars of Chinese chive (Allium tuberosum Rottl.). Plant Sci 119:149–156

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Myers JM, Simon PW (1998) Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets. Plant Cell Rep 17:726–730

    Article  CAS  Google Scholar 

  • Myers JM, Simon PW (1999) Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time. Plant Cell Rep 19:32–36

    Article  CAS  Google Scholar 

  • Robledo-Paz A, Vilalobos-Arambula VM, Jofre-Garfias AE (2000) Efficient plant regeneration of garlic (Allium sativum L.) by root-tip culture. In Vireo Cell Dev Plant 36:416–419

    Article  CAS  Google Scholar 

  • Song P, Peffley EB (1994) Plant regeneration from suspension cultures of Allium fistulosum and an A. fistulosum × A. cepa interspecific hybrid. Plant Sci 98:63–68

    Article  CAS  Google Scholar 

  • Štajner D, Popović BM (2009) Comparative study of antioxidant capacity in organs of different Allium species. Cent Eur J Biol 4:224–228

    Article  Google Scholar 

  • Štajner D, Čanadanović-Brunet J, Pavlović A (2004) Allium schoenoprasum L., as a natural antioxidant. Phytother Res 18:522–524

    Article  PubMed  Google Scholar 

  • Štajner D, Igić R, Popović BM, Dj Malenčić (2008) Comparative study of antioxidant properties of wild growing and cultivated Allium species. Phytother Res 22:113–117

    Article  PubMed  Google Scholar 

  • Van der Valk P, Scholten O, Verstappen F, Jansen R, Dons J (1992) High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species. Plant Cell Tiss Org Cult 30:181–192

    Article  Google Scholar 

  • Xu Z, Um YC, Kim CH, Lu G, Guo DP, Liu HL, Bah AA, Mao A (2008) Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation. Acta Physiol Plant 30:521–528

    Article  CAS  Google Scholar 

  • Yasseen MY, Barringer SA, Splittstoesser WE (1995) In vitro shoot proliferation and plant regeneration from kurrat (Allium ampeloprasum var. kurrat) seedlings. Plant Cell Tiss Org Cult 40:195–196

    Article  Google Scholar 

  • Zhang W, Lin X, Takano H, Takio S, Ono K (2004) Efficient plant regeneration from suspension cells of Allium cepa. Plant Cell Rep 23:371–376

    Article  CAS  PubMed  Google Scholar 

  • Zheng S, Henken B, Sofiari E, Jacobsen E, Krens FA, Kik C (1998) Factors influencing induction, propagation and regeneration of mature zygotic embryo-derived callus from Allium cepa. Plant Cell Tiss Org Cult 53:99–105

    Article  Google Scholar 

  • Zheng S, Henken B, Sofiari E, Keizer P, Jacobsen E, Kik C, Krens FA (1999) Effect of cytokinins and lines on plant regeneration from long-term callus and suspension cultures of Allium cepa L. Euphytica 108:83–90

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Ministry of Science and Technological Development of the Republic of Serbia, Contract No. 143026B.

Author information

Authors and Affiliations

  1. Institute for Biological Research “Siniša Stanković”, Boulevard Despota Stefana 142, 11000, Belgrade, Serbia

    S. Zdravković-Korać, J. Milojević, Lj. Tubić, D. Ćalić-Dragosavac, N. Mitić & B. Vinterhalter

Authors
  1. S. Zdravković-Korać
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Milojević
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lj. Tubić
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Ćalić-Dragosavac
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. Mitić
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. Vinterhalter
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Zdravković-Korać.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zdravković-Korać, S., Milojević, J., Tubić, L. et al. Somatic embryogenesis and plant regeneration from root sections of Allium schoenoprasum L.. Plant Cell Tiss Organ Cult 101, 237–244 (2010). https://doi.org/10.1007/s11240-010-9682-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-010-9682-z

Keywords

Search

Navigation