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Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation

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Abstract

In vitro shoot proliferation from stem disc of Allium chinense, a vegetatively propagated plant, was investigated in this experiment. In the present study, shoots were formed directly on stem discs on a medium containing 1 mg/l N6-benzyladenine (BA) and 0.5 mg/lα-naphthaleneacetic acid (NAA). These shoots were further cultured on MS media supplemented with various levels of BA in combination with NAA, and new shoot clusters developed easily from the explants cultured despite considerable differences in the induction of shoot clusters with different levels of BA and NAA. The most productive combination of growth regulators proved to be 1.0 mg/l BA and 1.0 mg/l NAA, in which about 17 shoots were produced per cluster in 8 weeks culture. Most of the formed shoots were rooted 15 days after being cultured on MS media supplemented with 0.1–1.0 mg/l NAA. The survival rate of the plantlets under ex vitro conditions was 95% in pots filled with a peat: sand (2:1 v/v) mixture after two weeks. In vitro bulblet formation were strongly promoted by the high temperature of 30°C compared to that at 25, 20 and 15°C, and 12% (w/v) sucrose appeared to be optimal for bulblet development. Results from this study demonstrated that A. chinense could be in vitro propagated by using stem discs and in vitro bulblet formation could be achieved.

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Abbreviations

BA:

N6-benzyladenine

MS:

Murashige and Skoog (1962) medium

NAA:

α-Naphthaleneacetic acid

PPFD:

Photosynthetic photon flux density

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Acknowledgments

This work was supported by a grant (No. 200618) from the Rural Development Administration, Republic of Korea. The author would like to thank Dr. S. J. Zheng from Wageningen University, The Netherlands, and Dr. R. B. Nan, for critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China

    Zhen Xu, Gang Lu, De-Ping Guo, Hai-Lin Liu & Amadou A. Bah

  2. Subtropical Horticulture Division, National Institute of Subtropical Agriculture, Rural Development Administration, Jeju, 690-150, Korea

    Yeong-Cheol Um & Chun-Hwan Kim

  3. Department of Environmental Sciences, University of Technology, Sydney NSW, 2000, Australia

    Aining Mao

Authors
  1. Zhen Xu
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  2. Yeong-Cheol Um
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  3. Chun-Hwan Kim
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  4. Gang Lu
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  5. De-Ping Guo
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  6. Hai-Lin Liu
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  7. Amadou A. Bah
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  8. Aining Mao
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Corresponding author

Correspondence to De-Ping Guo.

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Communicated by E. Lojkowska.

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Xu, Z., Um, YC., Kim, CH. et al. Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation. Acta Physiol Plant 30, 521–528 (2008). https://doi.org/10.1007/s11738-008-0150-x

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  • DOI: https://doi.org/10.1007/s11738-008-0150-x

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