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Somatic embryogenesis and plant regeneration in garden leek

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Abstract

High frequency plant regeneration via somatic embryogenesis has been induced from in vitro shoot-base cultures of seedlings of garden leek (Allium porrum L.). Four main steps are involved in the procedure using BDS medium:

  • - shoot multiplication with 17.6 mM benzyladenine;

  • - induction of nodular callus from the in vitro shoot base with 9 mM 2,4-dichlorophenoxyacetic acid;

  • - initiation of embryogenic callus from nodular callus with 9 mM 2,4-dichlorophenoxyacetic acid +7.6 mM abscisic acid;

  • - plant regeneration from embryogenic callus with 9.8 mM N6-(2-isopentenyl)adenine.

The presence of 2,4-dichlorophenoxyacetic acid in the medium and light conditions were shown to be essential for nodular callus induction and somatic embryogenesis. Abscisic acid was not a prerequiste for somatic embryogenesis, but it significantly increased the frequency.

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Abbreviations

ABA:

Abscisic acid

BA:

Benzyladenine

BDS:

Gamborg's B5 medium modified by Dunstan & Short (1977)

2iP:

N6-(2-isopeneny)adenine

2,4-d :

2,4-Dichlorophenoxyacetic acid

IBA:

Indole-3-butyric acid

TDZ:

Thidiazuron

BM:

Basal medium

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Authors and Affiliations

  1. Department of Plant Production-Horticulture, University Gent, Coupure links 653, B-9000, Gent, Belgium

    Wang Hong & Pierre Debergh

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  1. Wang Hong
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  2. Pierre Debergh
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Hong, W., Debergh, P. Somatic embryogenesis and plant regeneration in garden leek. Plant Cell Tiss Organ Cult 43, 21–28 (1995). https://doi.org/10.1007/BF00042667

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  • DOI: https://doi.org/10.1007/BF00042667

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